LOCUS pAAV.CaMKIIa.ChETA-EYFP.WPRE.hGH\(Mod\Addgene26969)\(p2355-Q) 7048 bp DNA circular 7-JUN-2011 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|591102206| COMMENT VNTDBDATE|592307148| COMMENT LSOWNER| COMMENT VNTNAME|pAAV.CaMKIIa.ChETA-EYFP.WPRE.hGH (Mod Addgene26969) (p2355-Q)| COMMENT VNTAUTHORNAME|Ruben Hovhannisyan| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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-809 100) COMMENT (CObjectList) (CObjectList)) COMMENT #223=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -739 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 17301638 COMMENT (CStringList) 1072745676 3538961 (CObList))) FEATURES Location/Qualifiers misc_feature 6372..6991 /vntifkey="21" /label=pBR322_origin misc_feature 4532..4838 /vntifkey="21" /label=f1_origin insertion_seq 3782..4260 /vntifkey="14" /label=hGH\polyA misc_marker 3166..3753 /vntifkey="22" /label=WPRE CDS 5357..6217 /vntifkey="4" /label=Amp-R promoter 145..1437 /vntifkey="29" /label=CamKII\promoter LTR complement(4300..4429) /vntifkey="19" /label=ITR LTR 1..130 /vntifkey="19" /label=ITR mutation 1844..1846 /vntifkey="62" /label=E123T CDS 1478..2422 /vntifkey="4" /label=ChR2(H134R/E123T) CDS 2423..3139 /vntifkey="4" /label=EYFP mutation 1844..1846 /vntifkey="62" /label=GAG\in\ChR2(H134R) BASE COUNT 1574 a 1903 c 1887 g 1684 t ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg 181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc 241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa 301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag 361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca 421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt 481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc 541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca 601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt 661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg 721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag 781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt 841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca 901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg 961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt 1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg 1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag 1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa 1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa 1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc 1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt 1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct 1441 agagcggtac cggatcctct agagtcgact agccaccatg gactatggcg gcgctttgtc 1501 tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg ggtccgtcct 1561 ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca cgaacggcgc 1621 tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt tgctgctgat 1681 gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct atgtgtgcgc 1741 cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc cctctatgct 1801 ctaccttgcc acaggacacc gggtgcagtg gctgcgctat gcaacctggc tgctcacttg 1861 tcctgtcatc cttatccgcc tgagcaacct caccggcctg agcaacgact acagcaggag 1921 aaccatggga ctccttgtct cagacatcgg gactatcgtg tggggggcta ccagcgccat 1981 ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga ttgtgctatg gcgcgaacac 2041 attttttcac gccgccaaag catatatcga gggttatcat actgtgccaa agggtcggtg 2101 ccgccaggtc gtgaccggca tggcatggct gtttttcgtg agctggggta tgttcccaat 2161 tctcttcatt ttggggcccg aaggttttgg cgtcctgagc gtctatggct ccaccgtagg 2221 tcacacgatt attgatctga tgagtaaaaa ttgttggggg ttgttgggac actacctgcg 2281 cgtcctgatc cacgagcaca tattgattca cggagatatc cgcaaaacca ccaaactgaa 2341 catcggcgga acggagatcg aggtcgagac tctcgtcgaa gacgaagccg aggccggagc 2401 cgtgccagcg gccgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 2461 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 2521 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 2581 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg 2641 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 2701 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 2761 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 2821 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 2881 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 2941 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 3001 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 3061 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 3121 ggacgagctg tacaagtaag aattcgatat caagcttatc gataatcaac ctctggatta 3181 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 3241 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 3301 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 3361 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 3421 cacctgtcag ctcctttccg gaactttcgc tttccccctc cctattgcca cggcggaact 3481 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 3541 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg 3601 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 3661 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 3721 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc 3781 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact 3841 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg 3901 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag 3961 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct 4021 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag 4081 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga 4141 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca 4201 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt 4261 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt 4321 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 4381 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 4441 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 4501 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 4561 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 4621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 4681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 4741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 4801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 4861 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 4921 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 4981 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 5041 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 5101 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 5161 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 5221 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 5281 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 5341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 5401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 5461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 5521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 5581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 5641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 5701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 5761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 5821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 5881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 5941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 6001 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 6061 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 6121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 6181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 6241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 6301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 6361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 6421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 6481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 6541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 6601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 6661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 6721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 6781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 6841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 6901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 6961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 7021 gccttttgct cacatgtcct gcaggcag //