LOCUS       pAAV.CaMKIIa.ChETA-EYFP.WPRE.hGH\(Mod\Addgene26969)\(p2355-Q)        7048 bp    DNA     circular      7-JUN-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|591102206|
COMMENT     VNTDBDATE|592307148|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CaMKIIa.ChETA-EYFP.WPRE.hGH (Mod Addgene26969) (p2355-Q)|
COMMENT     VNTAUTHORNAME|Ruben Hovhannisyan|
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FEATURES             Location/Qualifiers
     misc_feature    6372..6991
                     /vntifkey="21"
                     /label=pBR322_origin
     misc_feature    4532..4838
                     /vntifkey="21"
                     /label=f1_origin
     insertion_seq   3782..4260
                     /vntifkey="14"
                     /label=hGH\polyA
     misc_marker     3166..3753
                     /vntifkey="22"
                     /label=WPRE
     CDS             5357..6217
                     /vntifkey="4"
                     /label=Amp-R
     promoter        145..1437
                     /vntifkey="29"
                     /label=CamKII\promoter
     LTR             complement(4300..4429)
                     /vntifkey="19"
                     /label=ITR
     LTR             1..130
                     /vntifkey="19"
                     /label=ITR
     mutation        1844..1846
                     /vntifkey="62"
                     /label=E123T
     CDS             1478..2422
                     /vntifkey="4"
                     /label=ChR2(H134R/E123T)
     CDS             2423..3139
                     /vntifkey="4"
                     /label=EYFP
     mutation        1844..1846
                     /vntifkey="62"
                     /label=GAG\in\ChR2(H134R)
BASE COUNT     1574 a      1903 c      1887 g      1684 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg 
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc 
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa 
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag 
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca 
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt 
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc 
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca 
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt 
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg 
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag 
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt 
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca 
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg 
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt 
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg 
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag 
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa 
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa 
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc 
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt 
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct 
     1441 agagcggtac cggatcctct agagtcgact agccaccatg gactatggcg gcgctttgtc 
     1501 tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg ggtccgtcct 
     1561 ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca cgaacggcgc 
     1621 tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt tgctgctgat 
     1681 gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct atgtgtgcgc 
     1741 cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc cctctatgct 
     1801 ctaccttgcc acaggacacc gggtgcagtg gctgcgctat gcaacctggc tgctcacttg 
     1861 tcctgtcatc cttatccgcc tgagcaacct caccggcctg agcaacgact acagcaggag 
     1921 aaccatggga ctccttgtct cagacatcgg gactatcgtg tggggggcta ccagcgccat 
     1981 ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga ttgtgctatg gcgcgaacac 
     2041 attttttcac gccgccaaag catatatcga gggttatcat actgtgccaa agggtcggtg 
     2101 ccgccaggtc gtgaccggca tggcatggct gtttttcgtg agctggggta tgttcccaat 
     2161 tctcttcatt ttggggcccg aaggttttgg cgtcctgagc gtctatggct ccaccgtagg 
     2221 tcacacgatt attgatctga tgagtaaaaa ttgttggggg ttgttgggac actacctgcg 
     2281 cgtcctgatc cacgagcaca tattgattca cggagatatc cgcaaaacca ccaaactgaa 
     2341 catcggcgga acggagatcg aggtcgagac tctcgtcgaa gacgaagccg aggccggagc 
     2401 cgtgccagcg gccgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 
     2461 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 
     2521 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 
     2581 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg 
     2641 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 
     2701 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 
     2761 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 
     2821 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 
     2881 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 
     2941 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 
     3001 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 
     3061 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 
     3121 ggacgagctg tacaagtaag aattcgatat caagcttatc gataatcaac ctctggatta 
     3181 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     3241 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     3301 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     3361 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     3421 cacctgtcag ctcctttccg gaactttcgc tttccccctc cctattgcca cggcggaact 
     3481 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     3541 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg 
     3601 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     3661 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     3721 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc 
     3781 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact 
     3841 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg 
     3901 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag 
     3961 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct 
     4021 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag 
     4081 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga 
     4141 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca 
     4201 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt 
     4261 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt 
     4321 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     4381 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 
     4441 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 
     4501 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     4561 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     4621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     4681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 
     4741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     4801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 
     4861 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     4921 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 
     4981 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 
     5041 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 
     5101 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 
     5161 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     5221 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     5281 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     5341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     5401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     5461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     5521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     5581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     5641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     5701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     5761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     5821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     5881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     5941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     6001 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     6061 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     6121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     6181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     6241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     6301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     6361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     6421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     7021 gccttttgct cacatgtcct gcaggcag 
//
