LOCUS       pAAV.enCAG.hChR2(H134R)-mCherry.WPRE.SV40\(Addgene#\20938\MOD)-p1959        6939 bp    DNA     circular      9-SEP-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|564835398|
COMMENT     VNTDBDATE|564835483|
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FEATURES             Location/Qualifiers
     LTR             1..130
                     /vntifkey="19"
                     /label=5'\ITR
     misc_feature    438..719
                     /vntifkey="21"
                     /label=chicken\beta-actin\promoter
     enhancer        193..435
                     /vntifkey="9"
                     /label=CMV\enhancer
     intron          719..949
                     /vntifkey="15"
                     /label=chicken\beta-actin\intron
     misc_marker     3038..3625
                     /vntifkey="22"
                     /label=WPRE
     misc_feature    complement(4256..4711)
                     /vntifkey="21"
                     /label=f1\ori
     CDS             4842..5699
                     /vntifkey="4"
                     /label=AP(R)
     misc_feature    5873..6461
                     /vntifkey="21"
                     /label=Origin\of\replication
     repeat_region   complement(3950..4079)
                     /vntifkey="34"
                     /label=3'\ITR
     insertion_seq   3652..3880
                     /vntifkey="14"
                     /label=SV40\polyA
     CDS             1176..2105
                     /vntifkey="4"
                     /label=hChR2(H134R)
     intron          1029..1073
                     /vntifkey="15"
                     /label=Rabbit\beta-actin\intron
     CDS             2115..2822
                     /vntifkey="4"
                     /label=mCherry
BASE COUNT     1504 a      1853 c      1888 g      1694 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
      421 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
      481 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
      541 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg 
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat cgggatccac 
     1141 tagtccagtg tggtggaatt gcccttgctg ccaccatgga ctatggcggc gctttgtctg 
     1201 ccgtcggacg cgaacttttg ttcgttacta atcctgtggt ggtgaacggg tccgtcctgg 
     1261 tccctgagga tcaatgttac tgtgccggat ggattgaatc tcgcggcacg aacggcgctc 
     1321 agaccgcgtc aaatgtcctg cagtggcttg cagcaggatt cagcattttg ctgctgatgt 
     1381 tctatgccta ccaaacctgg aaatctacat gcggctggga ggagatctat gtgtgcgcca 
     1441 ttgaaatggt taaggtgatt ctcgagttct tttttgagtt taagaatccc tctatgctct 
     1501 accttgccac aggacaccgg gtgcagtggc tgcgctatgc agagtggctg ctcacttgtc 
     1561 ctgtcatcct tatccgcctg agcaacctca ccggcctgag caacgactac agcaggagaa 
     1621 ccatgggact ccttgtctca gacatcggga ctatcgtgtg gggggctacc agcgccatgg 
     1681 caaccggcta tgttaaagtc atcttctttt gtcttggatt gtgctatggc gcgaacacat 
     1741 tttttcacgc cgccaaagca tatatcgagg gttatcatac tgtgccaaag ggtcggtgcc 
     1801 gccaggtcgt gaccggcatg gcatggctgt ttttcgtgag ctggggtatg ttcccaattc 
     1861 tcttcatttt ggggcccgaa ggttttggcg tcctgagcgt ctatggctcc accgtaggtc 
     1921 acacgattat tgatctgatg agtaaaaatt gttgggggtt gttgggacac tacctgcgcg 
     1981 tcctgatcca cgagcacata ttgattcacg gagatatccg caaaaccacc aaactgaaca 
     2041 tcggcggaac ggagatcgag gtcgagactc tcgtcgaaga cgaagccgag gccggagccg 
     2101 tgccagcggc cgccgtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 
     2161 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 
     2221 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 
     2281 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 
     2341 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 
     2401 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 
     2461 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 
     2521 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 
     2581 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 
     2641 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 
     2701 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 
     2761 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 
     2821 aaagcggccc cggactcgag gccgcaggta agtatcaagg ttacaagaca ggtttaagga 
     2881 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct 
     2941 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcgagtg gagctcgcga 
     3001 ctagtcgatt cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 
     3061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 
     3121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 
     3181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 
     3241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 
     3301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 
     3361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 
     3421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 
     3481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 
     3541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 
     3601 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc cgggcggccg cttcgagcag 
     3661 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 
     3721 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 
     3781 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg 
     3841 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta 
     3901 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta 
     3961 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 
     4021 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 
     4081 cttaattaac ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     4141 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     4201 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 
     4261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     4321 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     4381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     4441 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     4501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     4561 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     4621 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     4681 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 
     4741 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     4801 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     4861 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     4921 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     4981 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     5041 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     5101 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     5161 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     5221 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     5281 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     5341 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     5401 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     5461 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     5521 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     5581 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     5641 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     5701 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 
     5761 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     5821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     5881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     5941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     6001 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     6061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     6121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     6181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     6241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     6301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     6361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     6421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     6481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     6541 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 
     6601 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 
     6661 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
     6721 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 
     6781 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 
     6841 ttcacacagg aaacagctat gaccatgatt acgccagatt taattaaggg caactgttgg 
     6901 gaagggcgat cggtgcgggc ctcttcgcta ttacgccag 
//
