LOCUS       Exported               10198 bp ds-DNA     circular SYN 20-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FOG[N+C] iCas9v2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 20, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    196..405
                     /label=CMV promoter
     misc_feature    529..552
                     /label=SV40 NLS1
     misc_feature    568..633
                     /label=3XFLAG
     misc_feature    664..798
                     /label=FOG1 aa1-45
     misc_feature    859..4962
                     /label=dCas9_D10A_H840A
     misc_feature    5014..5148
                     /label=FOG1 aa1-45
     misc_feature    5038..5085
                     /label=Nucleoplasmin NLS
                     /note="note=""direct strand 
                     translation""translation=""KRPAATKKAGQAKKKK"""
     misc_feature    5176..5223
                     /label=Nucleoplasmin NLS
     misc_feature    5242..5298
                     /label=P2A
     misc_feature    5349..5620
                     /label=TK polyA signal
     misc_feature    6099..6314
                     /label=SV40 enhancer-pBabe
     misc_feature    6278..6355
                     /label=SV40 ORI
     CDS             6497..7288
                     /label=Kan/neoR
     misc_binding    complement(7656..7678)
                     /label=LacO
     promoter        complement(7683..7712)
                     /label=lac
     rep_origin      complement(8018..8646)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(8798..9457)
                     /label=AmpR
     misc_feature    10106..195
                     /label=CAG enhancer
ORIGIN
        1 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
       61 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      121 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      481 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgcc caagaagaag
      541 aggaaggtgg gaggctccgg aggaagcgac tataaggacc acgacggaga ctacaaggat
      601 catgatattg attacaaaga cgatgacgat aagggtggcg ggtccggcgg tggatccggt
      661 accatgtcca ggcggaaaca gagcaacccc cggcagatca agcgttccct cggagacatg
      721 gaggccagag aggaggtgca gttggtgggt gccagccaca tggagcaaaa ggccacggca
      781 cctgaagccc cgagccctag taccggaggg agtggtggaa gcggcggttc tggtggctca
      841 ggcggcagcg gccggccaat ggacaagaag tactccattg ggctcgctat cggcacaaac
      901 agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
      961 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
     1021 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
     1081 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
     1141 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
     1201 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
     1261 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
     1321 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
     1381 aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag
     1441 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
     1501 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag
     1561 aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct
     1621 aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat
     1681 ctcgacaatc tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag
     1741 aacctgtcag acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa
     1801 gctccgctga gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg
     1861 ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag
     1921 tctaaaaatg gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa
     1981 tttattaagc ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac
     2041 agagaagatc tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt
     2101 cacctgggcg aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa
     2161 gataacaggg aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc
     2221 ctcgcccggg gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact
     2281 ccctggaact tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg
     2341 atgactaact ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg
     2401 tacgagtact tcacagttta taacgagctc accaaggtca aatacgtcac agaagggatg
     2461 agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag
     2521 acgaaccgga aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt
     2581 ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat
     2641 cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac
     2701 attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa
     2761 cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc
     2821 cgatatacag gatgggggcg gctgtcaaga aaactgatca atgggatccg agacaagcag
     2881 agtggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg
     2941 cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct
     3001 ggccaggggg acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa
     3061 aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat
     3121 aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag
     3181 aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa
     3241 atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac
     3301 tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc
     3361 gactacgacg tggatgctat cgtgccccag tcttttctca aagatgattc tattgataat
     3421 aaagtgttga caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa
     3481 gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa
     3541 cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc
     3601 ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt
     3661 ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa
     3721 gttattactc tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag
     3781 gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc
     3841 actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa
     3901 gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct
     3961 aagtacttct tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat
     4021 ggagagattc ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg
     4081 gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc
     4141 gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg
     4201 aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc
     4261 gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct
     4321 aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc
     4381 gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata aagaggtcaa aaaagacctc
     4441 atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc
     4501 gctagtgcgg gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat
     4561 ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag
     4621 aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc
     4681 gaattctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac
     4741 aataagcaca gggataagcc catcagggag caggcagaaa acattatcca cttgtttact
     4801 ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag
     4861 cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg
     4921 ctctatgaaa caagaatcga cctctctcag ctcggtggag acggtggtag tggaggttca
     4981 ggaggatccg gggggagcgg agggagcgct agcatgtcca ggcggaaaca gagcaacccc
     5041 cggcagatca agcgttccct cggagacatg gaggccagag aggaggtgca gttggtgggt
     5101 gccagccaca tggagcaaaa ggccacggca cctgaagccc cgagcccttc cggtggaggc
     5161 tccggaggtg gatctaaaag gccggcggcc acgaaaaagg ccggtcaggc aaaaaagaaa
     5221 aagggtggta gtggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     5281 gaggagaacc ctggacctgc ggccgcatga aagggttcga tccctaccgg ttagtaatga
     5341 gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5401 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     5461 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     5521 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     5581 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct
     5641 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5701 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5761 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt
     5821 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5881 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5941 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6001 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga
     6061 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6181 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6241 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6301 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6361 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6421 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     6481 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     6541 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     6601 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     6661 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6721 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6781 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6841 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6901 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6961 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7021 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7081 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7141 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7201 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7261 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc
     7321 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7381 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7441 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7561 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7621 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7681 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7741 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7801 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7861 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7921 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8221 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8461 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9781 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat
     9841 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9901 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9961 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    10021 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
    10081 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
    10141 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgcca
//
