LOCUS       Exported               10864 bp ds-DNA     circular SYN 20-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    DNMT3A-iCas9v2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 20, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    196..405
                     /label=CMV promoter
     misc_feature    529..552
                     /label=SV40 NLS1
     misc_feature    568..633
                     /label=3XFLAG
     misc_feature    670..1602
                     /label=Dnmt3a
     misc_feature    1663..5766
                     /label=dCas9_D10A_H840A
     misc_feature    5842..5889
                     /label=Nucleoplasmin NLS
                     /note="note=""direct strand 
                     translation""translation=""KRPAATKKAGQAKKKK"""
     misc_feature    5908..5964
                     /label=P2A
     misc_feature    6944..7021
                     /label=SV40 ORI
     CDS             7163..7954
                     /label=Kan/neoR
     misc_binding    complement(8322..8344)
                     /label=LacO
     promoter        complement(8349..8378)
                     /label=lac
     rep_origin      complement(8684..9312)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(9464..10123)
                     /label=AmpR
     misc_feature    10772..195
                     /label=CAG enhancer
ORIGIN
        1 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
       61 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      121 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      181 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      241 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      301 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      361 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      481 gaccgatcca gcctccggac tctagaggat cgaacccttg ccaccatgcc caagaagaag
      541 aggaaggtgg gaggctccgg aggaagcgac tataaggacc acgacggaga ctacaaggat
      601 catgatattg attacaaaga cgatgacgat aagggtggcg ggtccggcgg tggatccggt
      661 acccgcgccc cctcccggct ccagatgttc ttcgctaata accacgacca ggaatttgac
      721 cctccaaagg tttacccacc tgtcccagct gagaagagga agcccatccg ggtgctgtct
      781 ctctttgatg gaatcgctac agggctcctg gtgctgaagg acttgggcat tcaggtggac
      841 cgctacattg cctcggaggt gtgtgaggac tccatcacgg tgggcatggt gcggcaccag
      901 gggaagatca tgtacgtcgg ggacgtccgc agcgtcacac agaagcatat ccaggagtgg
      961 ggcccattcg atctggtgat tgggggcagt ccctgcaatg acctctccat cgtcaaccct
     1021 gctcgcaagg gcctctacga gggcactggc cggctcttct ttgagttcta ccgcctcctg
     1081 catgatgcgc ggcccaagga gggagatgat cgccccttct tctggctctt tgagaatgtg
     1141 gtggccatgg gcgttagtga caagagggac atctcgcgat ttctcgagtc caaccctgtg
     1201 atgattgatg ccaaagaagt gtcagctgca cacagggccc gctacttctg gggtaacctt
     1261 cccggtatga acaggccgtt ggcatccact gtgaatgata agctggagct gcaggagtgt
     1321 ctggagcatg gcaggatagc caagttcagc aaagtgagga ccattactac gaggtcaaac
     1381 tccataaagc agggcaaaga tcagcatttt cctgtcttca tgaatgagaa agaggacatc
     1441 ttatggtgca ctgaaatgga aagggtattt ggtttcccag tccactatac tgacgtctcc
     1501 aacatgagcc gcttggcgag gcagagactg ctgggccggt catggagcgt gccagtcatc
     1561 cgccacctct tcgctccgct gaaggagtat tttgcgtgtg tgagtaccgg agggagtggt
     1621 ggaagcggcg gttctggtgg ctcaggcggc agcggccggc caatggacaa gaagtactcc
     1681 attgggctcg ctatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag
     1741 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
     1801 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
     1861 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
     1921 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
     1981 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
     2041 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
     2101 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
     2161 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
     2221 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
     2281 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
     2341 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
     2401 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
     2461 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
     2521 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
     2581 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat
     2641 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
     2701 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
     2761 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
     2821 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
     2881 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
     2941 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
     3001 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
     3061 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc
     3121 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
     3181 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
     3241 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa
     3301 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
     3361 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
     3421 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
     3481 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
     3541 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
     3601 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
     3661 atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct taagtccgat
     3721 ggatttgcca accggaactt catgcagttg atccatgatg actctctcac ctttaaggag
     3781 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat
     3841 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
     3901 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
     3961 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
     4021 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
     4081 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
     4141 gaactggaca tcaatcggct ctccgactac gacgtggatg ctatcgtgcc ccagtctttt
     4201 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag
     4261 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
     4321 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
     4381 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
     4441 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
     4501 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc
     4561 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
     4621 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
     4681 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
     4741 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
     4801 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
     4861 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
     4921 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
     4981 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
     5041 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
     5101 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
     5161 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
     5221 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
     5281 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg
     5341 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
     5401 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
     5461 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
     5521 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
     5581 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
     5641 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
     5701 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
     5761 ggagacggtg gtagtggagg ttcaggagga tccgggggga gcggagggag cgctagcggt
     5821 ggaggctccg gaggtggatc taaaaggccg gcggccacga aaaaggccgg tcaggcaaaa
     5881 aagaaaaagg gtggtagtgg aagcggagct actaacttca gcctgctgaa gcaggctgga
     5941 gacgtggagg agaaccctgg acctgcggcc gcatgaaagg gttcgatccc taccggttag
     6001 taatgagttt aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     6061 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     6121 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     6181 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     6241 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct
     6301 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6361 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6421 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     6481 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6541 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6601 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6661 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     6721 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     6781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6841 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6901 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7021 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7081 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     7141 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7201 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7261 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7321 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7381 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7441 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7501 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7561 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7621 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7681 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7741 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7801 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7861 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7921 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga
     7981 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     8041 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8101 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8161 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8221 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8281 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8341 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8401 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8461 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8521 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8581 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8881 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9121 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9361 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9421 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9481 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9541 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9601 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9661 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9721 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9781 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9841 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9901 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9961 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10021 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10081 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10141 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10201 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10261 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10321 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10381 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10441 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag
    10501 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    10561 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    10621 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
    10681 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    10741 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10801 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    10861 gcca
//
