LOCUS Exported 8712 bp ds-DNA circular SYN 15-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSN20 complete_addgene deposit SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8712) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Created: Saturday, February 7, 2009 4:13 PM FEATURES Location/Qualifiers source 1..8712 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4137..4707 /note="Promoter hPGK" gene 4708..5424 /note="vex-GFP" misc_feature 5425..5490 /note="P2A" gene 5491..6570 /note="human OCT4 orf" misc_feature 6571..6771 /note="VP16 ctd..425-490" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg 4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcagctacgg cgtgcagtgc 4921 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4981 ggctacgtcc aggagcgcac catcagcttc aaggacgacg gcaactacaa gacccgcgcc 5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 5161 tatatcacgg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac 5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 5281 ggccccgtgc tgctgcccga caaccactac ctgttcatcc agtccgccct gagcaaagac 5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 5401 cacggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa 5461 gcaggtgacg tggaggagaa tcccggccct atggcgggac acctggcttc ggatttcgcc 5521 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt 5581 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg 5641 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt 5701 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg 5761 gagacctctc agcctgaggg tgaagcagga gtcggggtgg agagcaactc cgatggggcc 5821 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag 5881 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc 5941 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc 6001 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg 6061 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa 6121 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga 6181 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg 6241 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag 6301 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc 6361 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg 6421 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc 6481 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc 6541 accactctgg gctctcccat gcattcaaac cacttagacg gcgaggacgt ggcgatggcg 6601 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt 6661 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag 6721 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggtg agtcgacaat 6781 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6841 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6901 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6961 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 7021 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 7081 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 7141 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 7201 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 7261 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 7321 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct 7381 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 7441 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt 7501 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 7561 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 7621 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 7681 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 7741 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata 7801 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 7861 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag 7921 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 7981 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 8041 ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc 8101 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 8161 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 8221 caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc 8281 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 8341 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 8401 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 8461 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 8521 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 8581 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 8641 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 8701 tttacaattt cc //