LOCUS Exported 8508 bp ds-DNA circular SYN 15-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSN22 complete_addgene deposit SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8508) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Created: Monday, March 23, 2009 3:32 PM FEATURES Location/Qualifiers source 1..8508 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4137..4707 /note="Promoter hPGK" gene 4708..5424 /note="mCitrine" misc_feature 5425..5490 /note="P2A" gene 5491..6441 /note="hSOX2 orf" misc_feature 6442..6479 /note="1xF derived from VP16 ctd" misc_feature 6480..6519 /note="2xF derived from VP16 ctd" misc_feature 6520..6567 /note="3xF derived from VP16 ctd" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg 4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc 4921 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 5161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 5281 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac 5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 5401 ctcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa 5461 gcaggtgacg tggaggagaa tcccggccct atgtacaaca tgatggagac ggagctgaag 5521 ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca actccaccgc ggcggcggcc 5581 ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc ccatgaatgc cttcatggtg 5641 tggtcccgcg ggcagcggcg caagatggcc caggagaacc ccaagatgca caactcggag 5701 atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg agacggagaa gcggccgttc 5761 atcgacgagg ctaagcggct gcgagcgctg cacatgaagg agcacccgga ttataaatac 5821 cggccccggc ggaaaaccaa gacgctcatg aagaaggata agtacacgct gcccggcggg 5881 ctgctggccc ccggcggcaa tagcatggcg agcggggtcg gggtgggcgc cggcctgggc 5941 gcgggcgtga accagcgcat ggacagttac gcgcacatga acggctggag caacggcagc 6001 tacagcatga tgcaggacca gctgggctac ccgcagcacc cgggcctcaa tgcgcacggc 6061 gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg ccctgcagta caactccatg 6121 accagctcgc agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag 6181 ggcacccctg gcatggctct tggctccatg ggttcggtgg tcaagtccga ggccagctcc 6241 agcccccctg tggttacctc ttcctcccac tccagggcgc cctgccaggc cggggacctc 6301 cgggacatga tcagcatgta tctccccggc gccgaggtgc cggaacccgc cgcccccagc 6361 agacttcaca tgtcccagca ctaccagagc ggcccggtgc ccggcacggc cattaacggc 6421 acactgcccc tctcacacat gccggccgac gcccttgacg attttgactt agacatgctc 6481 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat 6541 tttgaccttg acatgctccc cgggtaagtc gacaatcaac ctctggatta caaaatttgt 6601 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 6661 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6721 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6781 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6841 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6961 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 7021 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 7081 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 7141 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt 7201 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 7261 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 7321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 7381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 7441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 7501 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 7561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 7621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 7681 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 7741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 7801 gaagtagtga ggaggctttt ttggaggcct aggcttttgc gtcgagacgt acccaattcg 7861 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 7921 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 7981 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 8041 gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 8101 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 8161 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 8221 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 8281 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 8341 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 8401 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 8461 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcc //