LOCUS       Exported                8955 bp ds-DNA     circular SYN 15-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSN25 complete_addgene deposit
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     Created: Monday, March 23, 2009 3:32 PM
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4137..4707
                     /note="Promoter hPGK"
     gene            4708..5424
                     /note="mCitrine"
     misc_feature    5425..5490
                     /note="P2A"
     gene            5491..6441
                     /note="hSOX2 orf"
     misc_feature    6442..7014
                     /note="HP1 alpha FL"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg
     4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc
     4921 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5281 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5401 ctcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa
     5461 gcaggtgacg tggaggagaa tcccggccct atgtacaaca tgatggagac ggagctgaag
     5521 ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca actccaccgc ggcggcggcc
     5581 ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc ccatgaatgc cttcatggtg
     5641 tggtcccgcg ggcagcggcg caagatggcc caggagaacc ccaagatgca caactcggag
     5701 atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg agacggagaa gcggccgttc
     5761 atcgacgagg ctaagcggct gcgagcgctg cacatgaagg agcacccgga ttataaatac
     5821 cggccccggc ggaaaaccaa gacgctcatg aagaaggata agtacacgct gcccggcggg
     5881 ctgctggccc ccggcggcaa tagcatggcg agcggggtcg gggtgggcgc cggcctgggc
     5941 gcgggcgtga accagcgcat ggacagttac gcgcacatga acggctggag caacggcagc
     6001 tacagcatga tgcaggacca gctgggctac ccgcagcacc cgggcctcaa tgcgcacggc
     6061 gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg ccctgcagta caactccatg
     6121 accagctcgc agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag
     6181 ggcacccctg gcatggctct tggctccatg ggttcggtgg tcaagtccga ggccagctcc
     6241 agcccccctg tggttacctc ttcctcccac tccagggcgc cctgccaggc cggggacctc
     6301 cgggacatga tcagcatgta tctccccggc gccgaggtgc cggaacccgc cgcccccagc
     6361 agacttcaca tgtcccagca ctaccagagc ggcccggtgc ccggcacggc cattaacggc
     6421 acactgcccc tctcacacat gggaaagaaa accaagcgga cagctgacag ttcttcttca
     6481 gaggatgagg aggagtatgt cgtggagaag gtgctagaca ggcgcgtggt taagggacaa
     6541 gtggaatatc tactgaagtg gaaaggcttt tctgaggagc acaatacttg ggaacctgag
     6601 aaaaacttgg attgccctga gctaatttct gaatttatga aaaagtataa gaagatgaag
     6661 gagggtgaaa ataataaacc cagggagaag tcagaaagta acaagaggaa atccaatttc
     6721 tcaaacagtg ccgatgacat caaatctaaa aaaaagagag agcagagcaa tgatatcgct
     6781 cggggctttg agagaggact ggaaccagaa aagatcattg gggcaacaga ttcctgtggt
     6841 gatttaatgt tcctaatgaa atggaaagac acagatgaag ctgacctggt tcttgcaaaa
     6901 gaagctaatg tgaaatgtcc acaaattgtg atagcatttt atgaagagag actgacatgg
     6961 catgcatatc ctgaggatgc ggaaaacaaa gagaaagaaa cagcaaagag ctaagtcgac
     7021 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7081 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7141 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7201 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7261 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7321 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7381 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7441 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7501 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7561 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     7621 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7681 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     7741 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7801 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7861 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7921 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7981 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8041 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8101 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8161 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8221 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8281 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8341 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8401 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8461 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
     8521 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8581 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8641 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8701 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8761 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8821 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8881 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8941 acgtttacaa tttcc
//
