LOCUS Exported 8844 bp ds-DNA circular SYN 15-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSN28 complete_addgene deposit SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8844) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Created: Monday, March 23, 2009 4:34 PM FEATURES Location/Qualifiers source 1..8844 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4137..4707 /note="Promoter hPGK" gene 4708..5424 /note="mCerulean" misc_feature 5425..5490 /note="P2A" gene 5491..6807 /note="human c-Myc orf" /note="human c-Myc orf" misc_feature 6808..6903 /note="VP16 ctd..425-455" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg 4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc 4921 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag cgacaacgtc 5161 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac 5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 5281 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac 5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 5401 ctcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa 5461 gcaggtgacg tggaggagaa tcccggccct atgcccctca acgttagctt caccaacagg 5521 aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac 5581 ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg 5641 aagaaattcg agctgctgcc caccccgccc ctgtccccta gccgccgctc cgggctctgc 5701 tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc 5761 gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg 5821 gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc 5881 caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc 5941 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc 6001 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac 6061 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg 6121 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc 6181 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc 6241 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg 6301 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct 6361 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca 6421 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc 6481 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc 6541 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta 6601 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc 6661 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag 6721 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa 6781 cttgaacagc tacggaactc ttgtgcgcac ttagacggcg aggacgtggc gatggcgcat 6841 gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg 6901 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 6961 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 7021 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 7081 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 7141 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 7201 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 7261 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 7321 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 7381 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 7441 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 7501 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 7561 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 7621 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 7681 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 7741 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 7801 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 7861 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 7921 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 7981 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 8041 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 8101 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 8161 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg 8221 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 8281 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 8341 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc 8401 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 8461 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 8521 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 8581 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 8641 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 8701 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 8761 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 8821 aaaatattaa cgtttacaat ttcc //