LOCUS       Exported                9321 bp ds-DNA     circular SYN 15-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSN29 complete_addgene deposit
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
COMMENT     Created: Monday, March 23, 2009 4:34 PM
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4137..4707
                     /note="Promoter hPGK"
     gene            4708..5424
                     /note="mCerulean"
     misc_feature    5425..5490
                     /note="P2A"
     gene            5491..6807
                     /note="human c-Myc orf"
                     /note="human c-Myc orf"
     misc_feature    6808..7380
                     /note="HP1 alpha FL"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg
     4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
     4921 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag cgacaacgtc
     5161 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5281 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5401 ctcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa
     5461 gcaggtgacg tggaggagaa tcccggccct atgcccctca acgttagctt caccaacagg
     5521 aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac
     5581 ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg
     5641 aagaaattcg agctgctgcc caccccgccc ctgtccccta gccgccgctc cgggctctgc
     5701 tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc
     5761 gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg
     5821 gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc
     5881 caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc
     5941 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc
     6001 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac
     6061 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg
     6121 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc
     6181 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc
     6241 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg
     6301 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct
     6361 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca
     6421 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc
     6481 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc
     6541 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta
     6601 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc
     6661 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag
     6721 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa
     6781 cttgaacagc tacggaactc ttgtgcggga aagaaaacca agcggacagc tgacagttct
     6841 tcttcagagg atgaggagga gtatgtcgtg gagaaggtgc tagacaggcg cgtggttaag
     6901 ggacaagtgg aatatctact gaagtggaaa ggcttttctg aggagcacaa tacttgggaa
     6961 cctgagaaaa acttggattg ccctgagcta atttctgaat ttatgaaaaa gtataagaag
     7021 atgaaggagg gtgaaaataa taaacccagg gagaagtcag aaagtaacaa gaggaaatcc
     7081 aatttctcaa acagtgccga tgacatcaaa tctaaaaaaa agagagagca gagcaatgat
     7141 atcgctcggg gctttgagag aggactggaa ccagaaaaga tcattggggc aacagattcc
     7201 tgtggtgatt taatgttcct aatgaaatgg aaagacacag atgaagctga cctggttctt
     7261 gcaaaagaag ctaatgtgaa atgtccacaa attgtgatag cattttatga agagagactg
     7321 acatggcatg catatcctga ggatgcggaa aacaaagaga aagaaacagc aaagagctaa
     7381 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7441 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7501 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7561 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7621 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7681 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7741 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7801 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7861 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7921 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     7981 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8041 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8101 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8161 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8221 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8281 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8341 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8461 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8521 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8581 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8641 cctaggcttt tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8701 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8761 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8821 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
     8881 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8941 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9001 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9061 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9121 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9181 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9241 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9301 atattaacgt ttacaatttc c
//
