LOCUS Exported 9405 bp ds-DNA circular SYN 15-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSN33 complete_addgene deposit SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9405) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Created: Monday, March 23, 2009 4:34 PM FEATURES Location/Qualifiers source 1..9405 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4137..4707 /note="Promoter hPGK" gene 4708..5415 /note="mCherry" misc_feature 5416..5481 /note="P2A" gene 5482..6891 /note="human Klf4 orf" /note="human Klf4 orf" misc_feature 6892..7464 /note="HP1 alpha FL" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagga taacatggcc 4741 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 4801 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 4861 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 4921 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 4981 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 5041 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 5101 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 5161 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 5221 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 5281 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 5341 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 5401 gacgagctgt acaagggatc tggagcaaca aacttctcac tactcaaaca agcaggtgac 5461 gtggaggaga atcccggccc tatggctgtc agcgacgcgc tgctcccatc tttctccacg 5521 ttcgcgtctg gcccggcggg aagggagaag acactgcgtc aagcaggtgc cccgaataac 5581 cgctggcggg aggagctctc ccacatgaag cgacttcccc cagtgcttcc cggccgcccc 5641 tatgacctgg cggcggcgac cgtggccaca gacctggaga gcggcggagc cggtgcggct 5701 tgcggcggta gcaacctggc gcccctacct cggagagaga ccgaggagtt caacgatctc 5761 ctggacctgg actttattct ctccaattcg ctgacccatc ctccggagtc agtggccgcc 5821 accgtgtcct cgtcagcgtc agcctcctct tcgtcgtcgc cgtcgagcag cggccctgcc 5881 agcgcgccct ccacctgcag cttcacctat ccgatccggg ccgggaacga cccgggcgtg 5941 gcgccgggcg gcacgggcgg aggcctcctc tatggcaggg agtccgctcc ccctccgacg 6001 gctcccttca acctggcgga catcaacgac gtgagcccct cgggcggctt cgtggccgag 6061 ctcctgcggc cagaattgga cccggtgtac attccgccgc agcagccgca gccgccaggt 6121 ggcgggctga tgggcaagtt cgtgctgaag gcgtcgctga gcgcccctgg cagcgagtac 6181 ggcagcccgt cggtcatcag cgtcagcaaa ggcagccctg acggcagcca cccggtggtg 6241 gtggcgccct acaacggcgg gccgccgcgc acgtgcccca agatcaagca ggaggcggtc 6301 tcttcgtgca cccacttggg cgctggaccc cctctcagca atggccaccg gccggctgca 6361 cacgacttcc ccctggggcg gcagctcccc agcaggacta ccccgaccct gggtcttgag 6421 gaagtgctga gcagcaggga ctgtcaccct gccctgccgc ttcctcccgg cttccatccc 6481 cacccggggc ccaattaccc atccttcctg cccgatcaga tgcagccgca agtcccgccg 6541 ctccattacc aagagctcat gccacccggt tcctgcatgc cagaggagcc caagccaaag 6601 aggggaagac gatcgtggcc ccggaaaagg accgccaccc acacttgtga ttacgcgggc 6661 tgcggcaaaa cctacacaaa gagttcccat ctcaaggcac acctgcgaac ccacacaggt 6721 gagaaacctt accactgtga ctgggacggc tgtggatgga aattcgcccg ctcagatgaa 6781 ctgaccaggc actaccgtaa acacacgggg caccgcccgt tccagtgcca aaaatgcgac 6841 cgagcatttt ccaggtcgga ccacctcgcc ttacacatga agaggcattt tggaaagaaa 6901 accaagcgga cagctgacag ttcttcttca gaggatgagg aggagtatgt cgtggagaag 6961 gtgctagaca ggcgcgtggt taagggacaa gtggaatatc tactgaagtg gaaaggcttt 7021 tctgaggagc acaatacttg ggaacctgag aaaaacttgg attgccctga gctaatttct 7081 gaatttatga aaaagtataa gaagatgaag gagggtgaaa ataataaacc cagggagaag 7141 tcagaaagta acaagaggaa atccaatttc tcaaacagtg ccgatgacat caaatctaaa 7201 aaaaagagag agcagagcaa tgatatcgct cggggctttg agagaggact ggaaccagaa 7261 aagatcattg gggcaacaga ttcctgtggt gatttaatgt tcctaatgaa atggaaagac 7321 acagatgaag ctgacctggt tcttgcaaaa gaagctaatg tgaaatgtcc acaaattgtg 7381 atagcatttt atgaagagag actgacatgg catgcatatc ctgaggatgc ggaaaacaaa 7441 gagaaagaaa cagcaaagag ctaagtcgac aatcaacctc tggattacaa aatttgtgaa 7501 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 7561 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 7621 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 7681 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 7741 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 7801 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 7861 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 7921 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7981 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 8041 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca 8101 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 8161 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 8221 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 8281 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 8341 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 8401 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 8461 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 8521 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 8581 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 8641 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 8701 gtagtgagga ggcttttttg gaggcctagg cttttgcgtc gagacgtacc caattcgccc 8761 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa 8821 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 8881 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 8941 tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 9001 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 9061 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 9121 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 9181 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 9241 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 9301 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 9361 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcc //