LOCUS Exported 9029 bp ds-DNA circular SYN 15-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSN35 complete_addgene deposit SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9029) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Created: Saturday, February 7, 2009 4:13 PM FEATURES Location/Qualifiers source 1..9029 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4137..4707 /note="Promoter hPGK" gene 4708..5424 /note="vexGFP" misc_feature 5425..5490 /note="P2A" gene 5491..6570 /note="human OCT4 orf" gene 6571..6705 /note="lambda linker" /note="lambda linker (92-136..45aa)" gene 6706..7046 /note="dEngrailed..168-281" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg 4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcagctacgg cgtgcagtgc 4921 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4981 ggctacgtcc aggagcgcac catcagcttc aaggacgacg gcaactacaa gacccgcgcc 5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 5161 tatatcacgg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac 5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 5281 ggccccgtgc tgctgcccga caaccactac ctgttcatcc agtccgccct gagcaaagac 5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 5401 cacggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa 5461 gcaggtgacg tggaggagaa tcccggccct atggcgggac acctggcttc ggatttcgcc 5521 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt 5581 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg 5641 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt 5701 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg 5761 gagacctctc agcctgaggg tgaagcagga gtcggggtgg agagcaactc cgatggggcc 5821 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag 5881 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc 5941 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc 6001 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg 6061 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa 6121 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga 6181 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg 6241 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag 6301 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc 6361 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg 6421 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc 6481 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc 6541 accactctgg gctctcccat gcattcaaac ggatccgtta gtatgcagcc gtcacttaga 6601 agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt 6661 agaaccttta ccaaaggtga tgcggagaga tgggtaagca gatccacggc caagccctcg 6721 ctggcctttt ccatctccaa catcctgagc gatcgtttcg gagatgtcca gaagcctggc 6781 aagtcgattg agaaccaggc cagcatattc cgccccttcg aggcgaatcg ctcccagact 6841 gccacgccct ccgcctttac aagagtggat ctgctggagt ttagccggca acagcaagct 6901 gccgccgcag ccgctactgc ggccatgatg ctggaacggg ccaacttcct taactgcttc 6961 aatccggctg cctatcccag gatacacgag gaaatcgtgc agagtcgcct gcgcaggagt 7021 gcagccaatg ccgtcatccc gcgcccagat ccactagttc tagaactata gtgagtcgta 7081 ttacgtaggt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 7141 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 7201 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 7261 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 7321 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 7381 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 7441 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 7501 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 7561 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 7621 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 7681 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 7741 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 7801 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 7861 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7921 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7981 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 8041 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 8101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 8161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 8221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 8281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 8341 tttggaggcc taggcttttg cgtcgagacg tacccaattc gccctatagt gagtcgtatt 8401 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 8461 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 8521 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct 8581 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 8641 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 8701 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 8761 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 8821 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 8881 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 8941 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 9001 ttaacaaaat attaacgttt acaatttcc //