LOCUS Exported 7686 bp ds-DNA circular SYN 15-AUG-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSN65 complete_addgene deposit SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7686) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Aug 15, 2017 from SnapGene 3.1.3 http://www.snapgene.com COMMENT Created: Monday, March 23, 2009 3:32 PM FEATURES Location/Qualifiers source 1..7686 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4137..4707 /note="Promoter hPGK" gene 4708..5424 /note="mCitrine" misc_feature 5425..5490 /note="P2A" gene 5494..5700 /note="SOX2 DBD (HMG box)" misc_feature 5701..5742 /note="1x V5 tag" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc caccggtatg gtgagcaagg gcgaggagct gttcaccggg 4741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 4801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 4861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc 4921 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 4981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 5041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 5101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 5161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac 5221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 5281 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac 5341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 5401 ctcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa 5461 gcaggtgacg tggaggagaa tcccggccct atggtcaagc ggcccatgaa tgccttcatg 5521 gtgtggtccc gcgggcagcg gcgcaagatg gcccaggaga accccaagat gcacaactcg 5581 gagatcagca agcgcctggg cgccgagtgg aaacttttgt cggagacgga gaagcggccg 5641 ttcatcgacg aggctaagcg gctgcgagcg ctgcacatga aggagcaccc ggattataaa 5701 ggcaagccca tccccaaccc cctgctgggc ctggactcca cctgagtcga caatcaacct 5761 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5821 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5881 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5941 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6001 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6061 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6121 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 6181 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6241 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 6301 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac 6361 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 6421 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 6481 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6541 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6601 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6661 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 6721 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 6781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 6841 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 6901 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 6961 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcgt 7021 cgagacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 7081 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 7141 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 7201 ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg 7261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 7321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 7381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 7441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 7501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 7561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 7621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 7681 atttcc //