LOCUS       pAAV-EF1a-EYFP-W        6067 bp    DNA     circular     20-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|538328968|
COMMENT     VNTDBDATE|538763081|
COMMENT     VNTNAME|pAAV-EF1a-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|William Hendriks|
COMMENT     VNTAUTHOREML|hendriks.william@mgh.harvard.edu|
COMMENT     VNTAUTHORNAME|Feng Zhang|
COMMENT     VNTAUTHOREML|zhang72@fas.harvard.edu|
FEATURES             Location/Qualifiers
     gene            1..141
                     /dnas_title="L-ITR"
                     /gene="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     gene            4269..4409
                     /dnas_title="R-ITR"
                     /gene="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     exon            4501..4807
                     /dnas_title="f1 Origin"
                     /gene="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     exon            5326..6183
                     /dnas_title="AmpR"
                     /gene="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6334..7001
                     /dnas_title="pUC ori"
                     /gene="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     misc_feature    150..155
                     /note="MluI"
     CDS             3098..4144
                     /label=Cre
     PCR_primer      3098..3122
                     /dnas_title="CCCAAGAAGAAGAGGAAGGTGTCCA"
                     /pair=""
                     /primer="CCCAAGAAGAAGAGGAAGGTGTCCA"
                     /current=0
     CDS             4145..4171
                     /dnas_title="HA"
                     /vntifkey="4"
                     /label=HA
     promoter        513..762
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     misc_feature    513..538
                     /note=""
     promoter        156..404
                     /dnas_title="Human U6 Promoter"
                     /vntifkey="29"
                     /label=Human U6 Promoter
     PCR_primer      complement(386..404)
                     /dnas_title="CGGTGTTTCGTCCTTTCCAC"
                     /pair=""
                     /primer="CGGTGTTTCGTCCTTTCCAC"
                     /current=0
     misc_feature    405..506
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    405..424
                     /note=""
     misc_feature    744..762
                     /note=""
     misc_feature    4193..4240
                     /note="shortPA"
     misc_feature    763..862
                     /note="chimeric RNA backbone 1 (SapI)"
     misc_feature    773..779
                     /note="SapI"
     misc_feature    764..770
                     /note="SapI"
     misc_feature    781..783
                     /note="3'"
     promoter        872..3082
                     /dnas_title="GFAP promoter"
                     /set="Set 1"
                     /vntifkey="29"
                     /label=GFAP promoter
                     /note="Unknown feature type:PCR_frame  Reading Frame"
     misc_feature    872..888
                     /note=""
     misc_feature    874..3082
                     /note="GFAP promotor"
     PCR_primer      874..895
                     /dnas_title="CCCACCTCCCTCTCTGTGCTGG"
                     /pair=""
                     /primer="CCCACCTCCCTCTCTGTGCTGG"
                     /current=0
     PCR_primer      complement(3066..3082)
                     /dnas_title="CCCGGCGAGCAGCGGAG"
                     /pair=""
                     /primer="CCCGGCGAGCAGCGGAG"
                     /current=0
     PCR_primer      complement(4154..4183)
                     /dnas_title="ATCGAATTCTTAAGCGTAATCTGGAACATC"
                     /pair=""
                     /primer="ATCGAATTCTTAAGCGTAATCTGGAACATC"
                     /current=0
     PCR_primer      complement(918..939)
                     /dnas_title="CATGTGATGGACAGACTGCCTC"
                     /pair=""
                     /primer="CATGTGATGGACAGACTGCCTC"
                     /current=0
     PCR_primer      3027..3045
                     /dnas_title="CGAGCAGAGCCAGAGCAGG"
                     /pair=""
                     /primer="CGAGCAGAGCCAGAGCAGG"
                     /current=0
     misc_feature    1973..1974
                     /note=""
     PCR_primer      complement(2025..2043)
                     /dnas_title="CATGAGCTGGCTGTGCTGG"
                     /pair=""
                     /primer="CATGAGCTGGCTGTGCTGG"
                     /current=0
     PCR_primer      1902..1923
                     /dnas_title="CATCCATCCCCAGAGGTTCTTC"
                     /pair=""
                     /primer="CATCCATCCCCAGAGGTTCTTC"
                     /current=0
     PCR_primer      complement(875..896)
                     /dnas_title="CCCAGCACAGAGAGGGAGGTGG"
                     /pair=""
                     /primer="CCCAGCACAGAGAGGGAGGTGG"
                     /current=1
     misc_feature    480..519
                     /note="pCM102-103_F1"
     misc_feature    397..430
                     /note="pCM102-103_F3"
     misc_feature    740..763
                     /note="readout_priming_site_F"
     misc_feature    729..763
                     /note="Readout_priming_site_long_F"
     misc_feature    897..936
                     /note="pCM103_R2"
     misc_feature    855..893
                     /note="pCM103_R1"
     source          1..7006
                     /dnas_title="pAAV-EF1a-EYFP-WPRE"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
      181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
      361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCGTGTAA TAGCTCCTGC
      421 ATGGgtttta gagctaGAAA tagcaagtta aaataaggct agtccgttat caacttgaaa
      481 aagtggcacc gagtcggtgc TTTTTTtcta gaagagggcc tatttcccat gattccttca
      541 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
      601 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      661 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      721 gatttcttgg ctttatatat cttGTGGAAA GGACGAAACA CCggaagagc gagctcttct
      781 gttttagagc taGAAAtagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      841 ggcaccgagt cggtgcTTTT TTggtaccag gatcccacct ccctctctgt gctgggactc
      901 acagagggag acctcaggag gcagtctgtc catcacatgt ccaaatgcag agcataccct
      961 gggctgggcg cagtggcgca caactgtaat tccagcactt tgggaggctg atgtggaagg
     1021 atcacttgag cccagaagtt ctagaccagc ctgggcaaca tggcaagacc ctatctctac
     1081 aaaaaaagtt aaaaaatcag ccacgtgtgg tgacacacac ctgtagtccc agctattcag
     1141 gaggctgagg tgaggggatc acttaaggct gggaggttga ggctgcagtg agtcgtggtt
     1201 gcgccactgc actccagcct gggcaacagt gagaccctgt ctcaaaagac aaaaaaaaaa
     1261 aaaaaaaaaa aaagaacata tcctggtgtg gagtagggga cgctgctctg acagaggctc
     1321 gggggcctga gctggctctg tgagctgggg aggaggcaga cagccaggcc ttgtctgcaa
     1381 gcagacctgg cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat
     1441 gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac
     1501 cccagccccc ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc
     1561 cagcctgaca gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg
     1621 ctgtgtggcg ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg
     1681 gatcagggga tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc
     1741 ttcccagaag tccaaggaca caaatgggtg aggggactgg gcagggttct gaccctgtgg
     1801 gaccagagtg gagggcgtag atggacctga agtctccagg gacaacaggg cccaggtctc
     1861 aggctcctag ttgggcccag tggctccagc gtttccaaac ccatccatcc ccagaggttc
     1921 ttcccatctc tccaggctga tgtgtgggaa ctcgaggaaa taaatctcca gtgggagacg
     1981 gaggggtggc cagggaaacg gggcgctgca ggaataaaga cgagccagca cagccagctc
     2041 atgtgtaacg gctttgtgga gctgtcaagg cctggtctct gggagagagg cacagggagg
     2101 ccagacaagg aaggggtgac ctggagggac agatccaggg gctaaagtcc tgataaggca
     2161 agagagtgcc ggccccctct tgccctatca ggacctccac tgccacatag aggccatgat
     2221 tgacccttag acaaagggct ggtgtccaat cccagccccc agccccagaa ctccagggaa
     2281 tgaatgggca gagagcagga atgtgggaca tctgtgttca agggaaggac tccaggagtc
     2341 tgctgggaat gaggcctagt aggaaatgag gtggcccttg agggtacaga acaggttcat
     2401 tcttcgccaa attcccagca ccttgcaggc acttacagct gagtgagata atgcctgggt
     2461 tatgaaatca aaaagttgga aagcaggtca gaggtcatct ggtacagccc ttccttccct
     2521 tttttttttt ttttttttgt gagacaaggt ctctctctgt tgcccaggct ggagtggcgc
     2581 aaacacagct cactgcagcc tcaacctact gggctcaagc aatcctccag cctcagcctc
     2641 ccaaagtgct gggattacaa gcatgagcca ccccactcag ccctttcctt cctttttaat
     2701 tgatgcataa taattgtaag tattcatcat ggtccaacca accctttctt gacccacctt
     2761 cctagagaga gggtcctctt gcttcagcgg tcagggcccc agacccatgg tctggctcca
     2821 ggtaccacct gcctcatgca ggagttggcg tgcccaggaa gctctgcctc tgggcacagt
     2881 gacctcagtg gggtgagggg agctctcccc atagctgggc tgcggcccaa ccccaccccc
     2941 tcaggctatg ccagggggtg ttgccagggg cacccgggca tcgccagtct agcccactcc
     3001 ttcataaagc cctcgcatcc caggagcgag cagagccaga gcaggatgga gaggagacgc
     3061 atcacctccg ctgctcgccg ggcgtacggc caccatgccc aagaagaaga ggaaggtgtc
     3121 caatctcctg actgttcacc agaacctccc tgcgctgcca gtagatgcca ctagcgatga
     3181 ggtcaggaaa aatctcatgg atatgtttag ggatagacag gcgttttctg aacacacctg
     3241 gaaaatgctg cttagcgtgt gccgatcctg ggcagcctgg tgtaagctga acaatcgcaa
     3301 atggttcccc gccgagccgg aggacgtgcg cgattacctg ctgtatctcc aggcaagagg
     3361 gctggctgtc aagactatcc agcagcactt gggccaactg aatatgctgc atcgacgcag
     3421 cgggctcccc cggcctagcg attcaaacgc agtctccctt gttatgagga gaattagaaa
     3481 ggaaaacgta gatgcgggtg agagggctaa gcaggctctc gcttttgagc ggactgattt
     3541 cgaccaggtc agatccctga tggagaacag cgatcggtgc caggacatca ggaacctcgc
     3601 atttctggga attgcatata acacacttct gcgcatagct gagatcgccc ggatcagagt
     3661 gaaagacatc agtcgaacgg acggcggccg gatgcttatt catattggac gcacaaagac
     3721 attggtcagc accgctggcg ttgaaaaggc cttgtccctg ggcgtaacga agctggtgga
     3781 aagatggatc tcagtgtccg gcgtggctga cgaccctaat aattacttgt tctgtcgagt
     3841 gagaaaaaac ggagtcgccg cgccctctgc caccagccaa ttgagtacac gggcccttga
     3901 agggatcttt gaggcaaccc accgactcat atacggagcc aaggatgaca gtggccagag
     3961 gtatctcgcc tggtcaggtc attctgctag ggtgggggcc gcacgagaca tggcgcgggc
     4021 aggagtctcc ataccagaga ttatgcaagc tggaggttgg acaaatgtga acatcgttat
     4081 gaactatatc cgcaatcttg actctgaaac cggggccatg gtgagactgc tcgaagatgg
     4141 tgactaccca tacgatgttc cagattacgc tTAAGAATTC gatatcaagc ttAATAAAAG
     4201 ATCTTTATTT TCATTAGATC TGTGTGTTGG TTTTTTGTGT ggtaaccacg tgcggaccga
     4261 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4321 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4381 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4441 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4501 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4561 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4621 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4681 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4741 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4801 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4861 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4921 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4981 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5041 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5101 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5161 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6481 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//

