LOCUS       Exported               11244 bp ds-DNA    circular SYN 25-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pEF1a-FB-dCas9-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 25, 2017 from SnapGene Viewer 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    277..298
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        313..343
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    351..367
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     375..391
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        417..627
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     promoter        439..635
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      486..621
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        727..1908
                     /note="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          957..1899
                     /note="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             1941..1964
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             2004..2048
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /note="AviTag(TM)"
                     /translation="GLNDIFEAQKIEWHE"
     CDS             2079..2099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             2100..6200
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6210..6230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     primer_bind     complement(6973..6989)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7071..7087)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7117..7135)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7156..7172)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7180..7196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7204..7234)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7249..7270
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7558..8146)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8317..9177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9178..9282)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      9309..9764
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     9905..9921
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9931..9949
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    10044..10268
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(10349..10948)
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     promoter        complement(10962..212)
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc
       61 gcgaaggggc caccaaagaa cggagccggt tggcgctacc ggtggatgtg gaatgtgtgc
      121 gaggccagag gccacttgtg tagcgccaag tgccagcggg gctgctaaag cgcatgctcc
      181 agactgcctt gggaaaagcg cctcccctac ccggtagaat tcgatctggc acgacaggtt
      241 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      301 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      361 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc tccccagcag
      421 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      481 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      541 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      601 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt
      661 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg
      721 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
      781 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      841 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      901 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      961 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     1021 gaattacttc cncgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     1081 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     1141 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     1201 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     1261 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     1321 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     1381 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     1441 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     1501 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     1561 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     1621 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     1681 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1741 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1801 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1861 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattgatcca
     1921 gatctttcga aatcaccatg gactacaaag acgatgacga taaagcaaga agagcatctg
     1981 tggaattcct gcagcccggg tccggcctga acgacatctt cgaggctcag aaaatcgaat
     2041 ggcacgaagg cgcgccgagc tcgaggatcc ttgctagccc caaaaagaag aggaaagtgg
     2101 acaagaagta ttctatcgga ctggccatcg ggactaatag cgtcgggtgg gccgtgatca
     2161 ctgacgagta caaggtgccc tctaagaagt tcaaggtgct cgggaacacc gaccggcatt
     2221 ccatcaagaa aaatctgatc ggagctctcc tctttgattc aggggagacc gctgaagcaa
     2281 cccgcctcaa gcggactgct agacggcggt acaccaggag gaagaaccgg atttgttacc
     2341 ttcaagagat attctccaac gaaatggcaa aggtcgacga cagcttcttc cataggctgg
     2401 aagaatcatt cctcgtggaa gaggataaga agcatgaacg gcatcccatc ttcggtaata
     2461 tcgtcgacga ggtggcctat cacgagaaat acccaaccat ctaccatctt cgcaaaaagc
     2521 tggtggactc aaccgacaag gcagacctcc ggcttatcta cctggccctg gcccacatga
     2581 tcaagttcag aggccacttc ctgatcgagg gcgacctcaa tcctgacaat agcgatgtgg
     2641 ataaactgtt catccagctg gtgcagactt acaaccagct ctttgaagag aaccccatca
     2701 atgcaagcgg agtcgatgcc aaggccattc tgtcagcccg gctgtcaaag agccgcagac
     2761 ttgagaatct tatcgctcag ctgccgggtg aaaagaaaaa tggactgttc gggaacctga
     2821 ttgctctttc acttgggctg actcccaatt tcaagtctaa tttcgacctg gcagaggatg
     2881 ccaagctgca actgtccaag gacacctatg atgacgatct cgacaacctc ctggcccaga
     2941 tcggtgacca atacgccgac cttttccttg ctgctaagaa tctttctgac gccatcctgc
     3001 tgtctgacat tctccgcgtg aacactgaaa tcaccaaggc ccctctttca gcttcaatga
     3061 ttaagcggta tgatgagcac caccaggacc tgaccctgct taaggcactc gtccggcagc
     3121 agcttccgga gaagtacaag gaaatcttct ttgaccagtc aaagaatgga tacgccggct
     3181 acatcgacgg aggtgcctcc caagaggaat tttataagtt tatcaaacct atccttgaga
     3241 agatggacgg caccgaagag ctcctcgtga aactgaatcg ggaggatctg ctgcggaagc
     3301 agcgcacttt cgacaatggg agcattcccc accagatcca tcttggggag cttcacgcca
     3361 tccttcggcg ccaagaggac ttctacccct ttcttaagga caacagggag aagattgaga
     3421 aaattctcac tttccgcatc ccctactacg tgggacccct cgccagagga aatagccggt
     3481 ttgcttggat gaccagaaag tcagaagaaa ctatcactcc ctggaacttc gaagaggtgg
     3541 tggacaaggg agccagcgct cagtcattca tcgaacggat gactaacttc gataagaacc
     3601 tccccaatga gaaggtcctg ccgaaacatt ccctgctcta cgagtacttt accgtgtaca
     3661 acgagctgac caaggtgaaa tatgtcaccg aagggatgag gaagcccgca ttcctgtcag
     3721 gcgaacaaaa gaaggcaatt gtggaccttc tgttcaagac caatagaaag gtgaccgtga
     3781 agcagctgaa ggaggactat ttcaagaaaa ttgaatgctt cgactctgtg gagattagcg
     3841 gggtcgaaga tcggttcaac gcaagcctgg gtacctacca tgatctgctt aagatcatca
     3901 aggacaagga ttttctggac aatgaggaga acgaggacat ccttgaggac attgtcctga
     3961 ctctcactct gttcgaggac cgggaaatga tcgaggagag gcttaagacc tacgcccatc
     4021 tgttcgacga taaagtgatg aagcaactta aacggagaag atataccgga tggggacgcc
     4081 ttagccgcaa actcatcaac ggaatccggg acaaacagag cggaaagacc attcttgatt
     4141 tccttaagag cgacggattc gctaatcgca acttcatgca acttatccat gatgattccc
     4201 tgacctttaa ggaggacatc cagaaggccc aagtgtctgg acaaggtgac tcactgcacg
     4261 agcatatcgc aaatctggct ggttcacccg ctattaagaa gggtattctc cagaccgtga
     4321 aagtcgtgga cgagctggtc aaggtgatgg gtcgccataa accagagaac attgtcatcg
     4381 agatggccag ggaaaaccag actacccaga agggacagaa gaacagcagg gagcggatga
     4441 aaagaattga ggaagggatt aaggagctcg ggtcacagat ccttaaagag cacccggtgg
     4501 aaaacaccca gcttcagaat gagaagctct atctgtacta ccttcaaaat ggacgcgata
     4561 tgtatgtgga ccaagagctt gatatcaaca ggctctcaga ctacgacgtg gacgccatcg
     4621 tccctcagag cttcctcaaa gacgactcaa ttgacaataa ggtgctgact cgctcagaca
     4681 agaaccgggg aaagtcagat aacgtgccct cagaggaagt cgtgaaaaag atgaagaact
     4741 attggcgcca gcttctgaac gcaaagctga tcactcagcg gaagttcgac aatctcacta
     4801 aggctgagag gggcggactg agcgaactgg acaaagcagg attcattaaa cggcaacttg
     4861 tggagactcg gcagattact aaacatgtcg cccaaatcct tgactcacgc atgaatacca
     4921 agtacgacga aaacgacaaa cttatccgcg aggtgaaggt gattaccctg aagtccaagc
     4981 tggtcagcga tttcagaaag gactttcaat tctacaaagt gcgggagatc aataactatc
     5041 atcatgctca tgacgcatat ctgaatgccg tggtgggaac cgccctgatc aagaagtacc
     5101 caaagctgga aagcgagttc gtgtacggag actacaaggt ctacgacgtg cgcaagatga
     5161 ttgccaaatc tgagcaggag atcggaaagg ccaccgcaaa gtacttcttc tacagcaaca
     5221 tcatgaattt cttcaagacc gaaatcaccc ttgcaaacgg tgagatccgg aagaggccgc
     5281 tcatcgagac taatggggag actggcgaaa tcgtgtggga caagggcaga gatttcgcta
     5341 ccgtgcgcaa agtgctttct atgcctcaag tgaacatcgt gaagaaaacc gaggtgcaaa
     5401 ccggaggctt ttctaaggaa tcaatcctcc ccaagcgcaa ctccgacaag ctcattgcaa
     5461 ggaagaagga ttgggaccct aagaagtacg gcggattcga ttcaccaact gtggcttatt
     5521 ctgtcctggt cgtggctaag gtggaaaaag gaaagtctaa gaagctcaag agcgtgaagg
     5581 aactgctggg tatcaccatt atggagcgca gctccttcga gaagaaccca attgactttc
     5641 tcgaagccaa aggttacaag gaagtcaaga aggaccttat catcaagctc ccaaagtata
     5701 gcctgttcga actggagaat gggcggaagc ggatgctcgc ctccgctggc gaacttcaga
     5761 agggtaatga gctggctctc ccctccaagt acgtgaattt cctctacctt gcaagccatt
     5821 acgagaagct gaaggggagc cccgaggaca acgagcaaaa gcaactgttt gtggagcagc
     5881 ataagcatta tctggacgag atcattgagc agatttccga gttttctaaa cgcgtcattc
     5941 tcgctgatgc caacctcgat aaagtcctta gcgcatacaa taagcacaga gacaaaccaa
     6001 ttcgggagca ggctgagaat atcatccacc tgttcaccct caccaatctt ggtgcccctg
     6061 ccgcattcaa gtacttcgac accaccatcg accggaaacg ctatacctcc accaaagaag
     6121 tgctggacgc caccctcatc caccagagca tcaccggact ttacgaaact cggattgacc
     6181 tctcacagct cggaggggat gagggagctc ccaagaaaaa gcgcaaggta ggtagttcct
     6241 aaactagttc tagatgaggg tccccacctg ggacccttga gagtatcagg tctcccacgt
     6301 gggagacaag aaatccctgt ttaatattta aacagcagtg ttccccatct gggtccttgc
     6361 acccctcact ctggcctcag ccgactgcac agcggcccct gcatcccctt ggctgtgagg
     6421 cccctggaca agcagaggtg gccagagctg ggaggcatgg ccctggggtc ccacgatcac
     6481 catgatttgc tggggaatct cgtttttctt cttaagactt ttgggacatg gtttgactcc
     6541 cgaacatcac cgacgtgtct cctgtttttc tgggtggcct cgggacacct gccctgcccc
     6601 cacgagggtc aggactgtga ctctttttag ggccaggcag gtgcctggac atttgccttg
     6661 ctggatgggg actggggatg tgggagggag cagacaggag gaatcatgtc aggcctgtgt
     6721 gtgaaaggaa gctccactgt caccctccac ctcttcaccc cccactcacc agtgtcccct
     6781 ccactgtcac attgtaactg aacttcagga taataaagtg tttgcctcca aaaaaaaaaa
     6841 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     6901 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     6961 aaaaaagaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7021 ccaacttaat cgccttgcag cacatccccc tttcgccaga tcaagcttat cgataccgtc
     7081 gacctcgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     7141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7981 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9301 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9361 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9601 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9661 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9721 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg
     9781 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9841 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9901 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag
     9961 ctccaccgcg gtggcggccg ctctagcctc gaggctagaa ctagtggatc tcgagcccca
    10021 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
    10081 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
    10141 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
    10201 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
    10261 aggcacagtc gaggctgatc agcgagctct acgatgcatg gggtcgtgcg ctcctttcgg
    10321 tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg
    10381 tcgcgcggtc cttcgggcac tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
    10441 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
    10501 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
    10561 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
    10621 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
    10681 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
    10741 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
    10801 gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt
    10861 agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg cgggtggcga
    10921 ggcgcaccgt gggcttgtac tcggtcatgg taagctggaa tcgaaaggcc cggagatgag
    10981 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggctccgg
    11041 aggaccttcg cgcccgcccc gcccctgagc ccgcccctga gcccgccccc ggacccaccc
    11101 cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa
    11161 aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt
    11221 gctacttcca tttgtcacgt cctg
//
