LOCUS       Exported                1853 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HC_Kan_conn_A-B
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    2..7
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    9..12
                     /label=A
                     /note="color: #ffff00"
     misc_feature    13..52
                     /label=conn A-B
                     /note="color: #c0c0c0"
     misc_feature    53..56
                     /label=B
                     /note="color: #ffff00"
     misc_feature    complement(58..63)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      130..161
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        162..264
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             265..1080
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1104..1131
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1143..1730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1752..1781
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 acgtctcgta ggtccactgg tgaggacgtg tgtgactatc tgactgttga ttatggcgag
       61 acgaagacga attctctaga tatcgctcaa tactgaccat ttaaatcata cctgacctcc
      121 atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt
      181 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
      241 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcgagg
      301 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      361 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
      421 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcaggcta
      481 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
      541 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa
      601 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
      661 tcgattcctg tttgtaattg tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg
      721 caatcacgaa tgaataacgg tttggttggt gcgagtgatt ttgatgacga gcgtaatggc
      781 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
      841 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
      901 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
      961 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     1021 attgataatc ctgatatgaa taaattgcag tttcacttga tgctcgatga gtttttctaa
     1081 tgagggccca aatgtaatca cctggctcac cttcgggtgg gcctttctgc gttgctggcg
     1141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgatgctc aagtcagagg
     1201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1621 acctcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1741 tgattttcta ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg attgcagtcc
     1801 agttacgctg gagtctgagg ctcgtcctga atgatatcaa gcttgaattc gtt
//
