LOCUS Exported 1943 bp ds-DNA circular SYN 10-JUL-2017 DEFINITION High-copy number vector with a kanamycin resistance marker for efficient blunt cloning of unstable sequences. ACCESSION AF532107 VERSION . KEYWORDS pSMART HCKan SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1943) AUTHORS Lucigen TITLE Direct Submission JOURNAL Exported Friday, Jul 21, 2017 from SnapGene 3.3.4 http://www.snapgene.com COMMENT Linearize at HincII to make blunt cloning vector. FEATURES Location/Qualifiers source 1..1943 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 79..84 /label=BsmBI /note="color: #00ffff; direction: RIGHT" misc_feature 86..89 /label=A /note="color: #ffff00" misc_feature 90..181 /label=con_A-C /note="color: #a6acb3" misc_feature 182..185 /label=C /note="color: #ffff00" misc_feature complement(187..192) /label=BsmBI /note="color: #00ffff; direction: LEFT" terminator 286..315 /label=T3Te terminator /note="phage T3 early transcription terminator" /note="color: #ff0000" rep_origin 337..924 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" terminator 936..963 /label=T7Te terminator /note="phage T7 early transcription terminator" /note="color: #ff0000" CDS complement(987..1802) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter complement(1803..1905) /label=cat promoter /note="promoter of the E. coli cat gene" /note="color: #ffffff; direction: LEFT" terminator 1906..1937 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" /note="color: #ff0000" ORIGIN 1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtctg 61 catccacgat ctagtcaccg tctcgtaggt cttgtattca gtctcgcact ttagacatgg 121 tcttctgctg cccgtggtcc tctattcgtc ggatgttctg tcgattcttc ctgtctatct 181 ggactcgaga cgctactcac cctcgagcat aacaacgaat tcaagcttga tatcattcag 241 gacgagcctc agactccagc gtaactggac tgcaatcaac tcactggctc accttcacgg 301 gtgggccttt cttcggtaga aaatcaaagg atcttcttga gatccttttt ttctgcgcgt 361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 421 agagctacca actctttttc cgaggtaact ggcttcagca gagcgcagat accaaatact 481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc atcgattttt gtgatgctcg 901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga 961 gccaggtgat tacatttggg ccctcattag aaaaactcat cgagcatcaa gtgaaactgc 1021 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa 1081 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt 1141 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca 1201 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt 1261 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcacca 1321 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgccgtta 1381 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca 1441 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg 1501 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga 1561 agaggcataa attccgtcag ccagtttagc ctgaccatct catctgtaac atcattggca 1621 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga 1681 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca 1741 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc 1801 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat 1861 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaagtca aaagcctccg 1921 gtcggaggct tttgactttc tgc //