LOCUS       Exported                1943 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    79..84
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    86..89
                     /label=A
                     /note="color: #ffff00"
     misc_feature    90..181
                     /label=con_A-C
                     /note="color: #a6acb3"
     misc_feature    182..185
                     /label=C
                     /note="color: #ffff00"
     misc_feature    complement(187..192)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      286..315
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      337..924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      936..963
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(987..1802)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1803..1905)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      1906..1937
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtctg
       61 catccacgat ctagtcaccg tctcgtaggt cttgtattca gtctcgcact ttagacatgg
      121 tcttctgctg cccgtggtcc tctattcgtc ggatgttctg tcgattcttc ctgtctatct
      181 ggactcgaga cgctactcac cctcgagcat aacaacgaat tcaagcttga tatcattcag
      241 gacgagcctc agactccagc gtaactggac tgcaatcaac tcactggctc accttcacgg
      301 gtgggccttt cttcggtaga aaatcaaagg atcttcttga gatccttttt ttctgcgcgt
      361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      421 agagctacca actctttttc cgaggtaact ggcttcagca gagcgcagat accaaatact
      481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc atcgattttt gtgatgctcg
      901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga
      961 gccaggtgat tacatttggg ccctcattag aaaaactcat cgagcatcaa gtgaaactgc
     1021 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     1081 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     1141 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     1201 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     1261 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcacca
     1321 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgccgtta
     1381 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     1441 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg
     1501 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     1561 agaggcataa attccgtcag ccagtttagc ctgaccatct catctgtaac atcattggca
     1621 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     1681 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     1741 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc
     1801 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1861 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaagtca aaagcctccg
     1921 gtcggaggct tttgactttc tgc
//
