LOCUS       Exported                1855 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HC_Kan_conn_E-F
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    2..7
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    9..12
                     /label=E
                     /note="color: #ffff00"
     misc_feature    13..54
                     /label=conn E-F
                     /note="color: #c0c0c0"
     misc_feature    55..58
                     /label=F
                     /note="color: #ffff00"
     misc_feature    complement(60..65)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      132..163
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        164..266
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             267..1082
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1106..1133
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1145..1732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1754..1783
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 acgtctcgtc cgttagcctt tagtgaaccg tcagaattaa ttcagatcga tctaccagcg
       61 agacgaagac gaattctcta gatatcgctc aatactgacc atttaaatca tacctgacct
      121 ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag
      181 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
      241 gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgctcga
      301 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      361 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      421 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcaggc
      481 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      541 atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag
      601 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      661 attcgattcc tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg
      721 cgcaatcacg aatgaataac ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg
      781 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
      841 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      901 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
      961 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1021 gtattgataa tcctgatatg aataaattgc agtttcactt gatgctcgat gagtttttct
     1081 aatgagggcc caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg
     1141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga
     1201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1561 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1621 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1681 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1741 tttgattttc taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgcagt
     1801 ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat tcgtt
//
