LOCUS       Exported                1892 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    78..83
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    85..88
                     /label=D
                     /note="color: #ffff00"
     misc_feature    89..130
                     /label=con_D-F
                     /note="color: #a6acb3"
     misc_feature    131..134
                     /label=F
                     /note="color: #ffff00"
     misc_feature    complement(136..141)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      234..263
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      285..872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      884..911
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(935..1750)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1751..1853)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      1854..1885
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 atggaggtca ggtatgattt aaatggtcag tattgagcga tatctagaga attcgtcaga
       61 agttggtgag ttcagagcgt ctcaggactt agcctttagt gaaccgtcag aattaattca
      121 gatcgatcta ccagcgagac ggtgaatgag gagattgagc taacgaattc aagcttgata
      181 tcattcagga cgagcctcag actccagcgt aactggactg caatcaactc actggctcac
      241 cttcacgggt gggcctttct tcggtagaaa atcaaaggat cttcttgaga tccttttttt
      301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      361 ccggatcaag agctaccaac tctttttccg aggtaactgg cttcagcaga gcgcagatac
      421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcat cgatttttgt
      841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcagaa aggcccaccc
      901 gaaggtgagc caggtgatta catttgggcc ctcattagaa aaactcatcg agcatcaagt
      961 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     1021 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     1081 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     1141 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     1201 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     1261 tcgcaccaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     1321 cgccgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     1381 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     1441 tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     1501 tggtcggaag aggcataaat tccgtcagcc agtttagcct gaccatctca tctgtaacat
     1561 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     1621 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     1681 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     1741 tatggctcat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1801 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaagtcaaa
     1861 agcctccggt cggaggcttt tgactttctg ct
//
