LOCUS Exported 1871 bp ds-DNA circular SYN 02-AUG-2016 DEFINITION . ACCESSION . VERSION . KEYWORDS SnapGene DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1871) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Friday, Jul 21, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1871 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 77..82 /label=BsmBI /note="color: #a6acb3; direction: RIGHT" misc_feature 84..87 /label=B /note="color: #ffff00" misc_feature 88..127 /label=conn B-E /note="color: #c0c0c0" misc_feature 128..131 /label=E /note="color: #ffff00" misc_feature complement(133..138) /label=BsmBI /note="color: #a6acb3; direction: LEFT" terminator 213..244 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" /note="color: #ff0000" promoter 245..347 /label=cat promoter /note="promoter of the E. coli cat gene" /note="color: #ffffff; direction: RIGHT" CDS 348..1163 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" terminator 1187..1214 /label=T7Te terminator /note="phage T7 early transcription terminator" /note="color: #ff0000" rep_origin complement(1226..1813) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" terminator 1835..1864 /label=T3Te terminator /note="phage T3 early transcription terminator" /note="color: #ff0000" ORIGIN 1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat 61 tcgtttttaa cgcagccgtc tccatggact aactacggtg aaagagactt agaaggagat 121 tgagagatcc gtgagacgag gccgttatga cgaattctct agatatcgct caatactgac 181 catttaaatc atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt 241 gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg 301 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc 361 aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg 421 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg 481 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 541 ttacagatga gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca 601 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa 661 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 721 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc 781 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg 841 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc 901 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 961 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 1021 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 1081 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact 1141 tgatgctcga tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg 1201 tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 1261 aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 1681 tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa 1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 1801 aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg 1861 agccagtgag t //