LOCUS Exported 1899 bp ds-DNA circular SYN 10-JUL-2017 DEFINITION High-copy number vector with a kanamycin resistance marker for efficient blunt cloning of unstable sequences. ACCESSION AF532107 VERSION . KEYWORDS pSMART HCKan SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1899) AUTHORS Lucigen TITLE Direct Submission JOURNAL Exported Friday, Jul 21, 2017 from SnapGene 3.3.4 http://www.snapgene.com COMMENT Linearize at HincII to make blunt cloning vector. FEATURES Location/Qualifiers source 1..1899 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 80..85 /label=BsmBI /note="color: #00ffff; direction: RIGHT" misc_feature 87..90 /label=H /note="color: #ffff00" misc_feature 91..138 /label=conn H-K /note="color: #a6acb3" CDS 91..93 /codon_start=1 /label=STOP /note="color: #993366" /translation="" misc_feature 139..142 /label=K /note="color: #ffff00" misc_feature complement(144..149) /label=BsmBI /note="color: #00ffff; direction: LEFT" terminator 243..272 /label=T3Te terminator /note="phage T3 early transcription terminator" /note="color: #ff0000" rep_origin 294..881 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" terminator 893..920 /label=T7Te terminator /note="phage T7 early transcription terminator" /note="color: #ff0000" CDS complement(944..1759) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter complement(1760..1862) /label=cat promoter /note="promoter of the E. coli cat gene" /note="color: #ffffff; direction: LEFT" terminator 1863..1894 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" /note="color: #ff0000" ORIGIN 1 ctatggaggt caggtatgat ttaaatggtc agtattgagc gatatctaga gaattcgtct 61 gcatccacga tctagtcacc gtctcaaggc taataacagc ttccggactc tagaacatcc 121 ctacaggtga tatcctcggg tacgagacgc tactcaccct cgagcataac aacgaattca 181 agcttgatat cattcaggac gagcctcaga ctccagcgta actggactgc aatcaactca 241 ctggctcacc ttcacgggtg ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat 301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 361 gtttgtttgc cggatcaaga gctaccaact ctttttccga ggtaactggc ttcagcagag 421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcatc 841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcagaaa 901 ggcccacccg aaggtgagcc aggtgattac atttgggccc tcattagaaa aactcatcga 961 gcatcaagtg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa 1021 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct 1081 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt 1141 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg 1201 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat 1261 caaaatcact cgcaccaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa 1321 atacgcgatc gccgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga 1381 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga 1441 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa 1501 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagcctg accatctcat 1561 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg 1621 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt 1681 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt 1741 cccgttgaat atggctcatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa 1801 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat 1861 caagtcaaaa gcctccggtc ggaggctttt gactttctg //