LOCUS       Exported                1905 bp ds-DNA     circular SYN 03-NOV-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    2..7
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    9..12
                     /label=L
                     /note="color: #ffff00"
     misc_feature    13..104
                     /label=conn L-M
                     /note="color: #a6acb3"
     misc_feature    105..108
                     /label=M
                     /note="color: #ffff00"
     misc_feature    complement(110..115)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      182..213
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        214..316
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             317..1132
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1156..1183
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1195..1782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1804..1833
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 acgtctcgcg tctatgatga ggatgttggt ggagagcatg tggaggaagt ggatagggaa
       61 ggttgtagag tagatccggt tgaagtgatg aggataggag gaggtcaccg agacgaagac
      121 gaattctcta gatatcgctc aatactgacc atttaaatca tacctgacct ccatagcaga
      181 aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt
      241 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      301 agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
      361 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
      421 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
      481 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct
      541 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
      601 gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga
      661 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
      721 tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
      781 aatgaataac ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt
      841 tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac
      901 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
      961 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     1021 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     1081 tcctgatatg aataaattgc agtttcactt gatgctcgat gagtttttct aatgagggcc
     1141 caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg cgtttttcca
     1201 taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa
     1261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttacctcgga
     1681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgattttc
     1801 taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgcagt ccagttacgc
     1861 tggagtctga ggctcgtcct gaatgatatc aagcttgaat tcgtt
//
