LOCUS       Exported                1942 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    77..82
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    84..87
                     /label=L
                     /note="color: #ffff00"
     misc_feature    88..179
                     /label=conn_L-N
                     /note="color: #a6acb3"
     misc_feature    180..183
                     /label=N
                     /note="color: #ffff00"
     misc_feature    complement(185..190)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      283..312
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      334..921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      933..960
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(984..1799)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1800..1902)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      1903..1934
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtcagaa
       61 gttggtgagt tcagagcgtc tcacgtctat gatgaggatg ttggtggaga gcatgtggag
      121 gaagtggata gggaaggttg tagagtagat ccggttgaag tgatgaggat aggaggaggc
      181 taccgagacg gtgaatgagg agattgagct aacgaattca agcttgatat cattcaggac
      241 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg
      301 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      421 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      841 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca
      901 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc
      961 aggtgattac atttgggccc tcattagaaa aactcatcga gcatcaagtg aaactgcaat
     1021 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     1081 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     1141 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     1201 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     1261 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcaccaacc
     1321 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa
     1381 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     1441 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt ccctgggatc
     1501 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     1561 ggcataaatt ccgtcagcca gtttagcctg accatctcat ctgtaacatc attggcaacg
     1621 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     1681 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     1741 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
     1921 ggaggctttt gactttctgc ta
//
