LOCUS Exported 1943 bp ds-DNA circular SYN 10-JUL-2017 DEFINITION High-copy number vector with a kanamycin resistance marker for efficient blunt cloning of unstable sequences. ACCESSION AF532107 VERSION . KEYWORDS pSMART HCKan SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1943) AUTHORS Lucigen TITLE Direct Submission JOURNAL Exported Friday, Jul 21, 2017 from SnapGene 3.3.4 http://www.snapgene.com COMMENT Linearize at HincII to make blunt cloning vector. FEATURES Location/Qualifiers source 1..1943 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 86..91 /label=BsmBI /note="color: #00ffff; direction: RIGHT" misc_feature 93..96 /label=L /note="color: #ffff00" misc_feature 97..188 /label=conn L-R /note="color: #a6acb3" misc_feature 189..192 /label=R /note="color: #ffff00" misc_feature complement(194..199) /label=BsmBI /note="color: #00ffff; direction: LEFT" terminator 285..316 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" /note="color: #ff0000" promoter 317..419 /label=cat promoter /note="promoter of the E. coli cat gene" /note="color: #ffffff; direction: RIGHT" CDS 420..1235 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" terminator 1259..1286 /label=T7Te terminator /note="phage T7 early transcription terminator" /note="color: #ff0000" rep_origin complement(1298..1885) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" terminator 1907..1936 /label=T3Te terminator /note="phage T3 early transcription terminator" /note="color: #ff0000" ORIGIN 1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat 61 tcgtttgcat ccacgatcta gtcaccgtct cacgtctatg atgaggatgt tggtggagag 121 catgtggagg aagtggatag ggaaggttgt agagtagatc cggttgaagt gatgaggata 181 ggaggaggcg gtcgagacgc tactcaccct cgagcataac gacgaattct ctagatatcg 241 ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga 301 ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa 361 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa 421 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg 481 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 541 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 601 ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc 661 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 721 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 781 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 841 ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 901 ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 961 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 1021 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 1081 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 1141 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 1201 tgcagtttca cttgatgctc gatgagtttt tctaatgagg gcccaaatgt aatcacctgg 1261 ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg 1321 acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa 1381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 1561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 1621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 1681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 1741 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc 1801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca 1921 cccgtgaagg tgagccagtg agt //