LOCUS       Exported                1943 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    86..91
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    93..96
                     /label=L
                     /note="color: #ffff00"
     misc_feature    97..188
                     /label=conn L-R
                     /note="color: #a6acb3"
     misc_feature    189..192
                     /label=R
                     /note="color: #ffff00"
     misc_feature    complement(194..199)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      285..316
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        317..419
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             420..1235
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1259..1286
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1298..1885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1907..1936
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttgcat ccacgatcta gtcaccgtct cacgtctatg atgaggatgt tggtggagag
      121 catgtggagg aagtggatag ggaaggttgt agagtagatc cggttgaagt gatgaggata
      181 ggaggaggcg gtcgagacgc tactcaccct cgagcataac gacgaattct ctagatatcg
      241 ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga
      301 ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
      361 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      421 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      481 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      541 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      601 ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc
      661 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      721 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      781 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      841 ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      901 ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      961 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1021 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1081 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1141 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1201 tgcagtttca cttgatgctc gatgagtttt tctaatgagg gcccaaatgt aatcacctgg
     1261 ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg
     1321 acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1741 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc
     1801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca
     1921 cccgtgaagg tgagccagtg agt
//
