LOCUS       Exported                1943 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    21..26
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    28..31
                     /label=L
                     /note="color: #ffff00"
     misc_feature    32..123
                     /label=conn. L-W
                     /note="color: #a6acb3"
     misc_feature    124..127
                     /label=W
                     /note="color: #ffff00"
     misc_feature    complement(129..134)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      220..251
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        252..354
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             355..1170
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1194..1221
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1233..1820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1842..1871
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tgcatccacg atctagtcac cgtctcacgt ctatgatgag gatgttggtg gagagcatgt
       61 ggaggaagtg gatagggaag gttgtagagt agatccggtt gaagtgatga ggataggagg
      121 aggcgaacga gacgctactc accctcgagc ataacgacga attctctaga tatcgctcaa
      181 tactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga
      241 ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
      301 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc
      361 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
      421 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      481 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      541 aatgatgtta cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg
      601 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca
      661 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      721 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      781 ggcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttggt
      841 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      901 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      961 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1021 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1081 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1141 tttcacttga tgctcgatga gtttttctaa tgagggccca aatgtaatca cctggctcac
     1201 cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1261 catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1321 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1381 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1441 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1501 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1561 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1621 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1681 tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat
     1741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1801 gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccgaagaaag gcccacccgt
     1861 gaaggtgagc cagtgagttg attgcagtcc agttacgctg gagtctgagg ctcgtcctga
     1921 atgatatcaa gcttgaattc gtt
//
