LOCUS       Exported                1871 bp ds-DNA     circular SYN 17-MAY-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    77..82
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    84..87
                     /label=R
                     /note="color: #ffff00"
     misc_feature    88..127
                     /label=conn R-W
                     /note="color: #c0c0c0"
     misc_feature    128..131
                     /label=W
                     /note="color: #ffff00"
     misc_feature    complement(133..138)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      213..244
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        245..347
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             348..1163
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1187..1214
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1226..1813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1835..1864
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tcccggtaga ggcgaaggaa cgacaacaag agcggacaac
      121 acagacacga atgagacgag gccgttatga cgaattctct agatatcgct caatactgac
      181 catttaaatc atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt
      241 gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      301 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc
      361 aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg
      421 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
      481 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      541 ttacagatga gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      601 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa
      661 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      721 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc
      781 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg
      841 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
      901 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      961 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     1021 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     1081 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact
     1141 tgatgctcga tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg
     1201 tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1261 aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1681 tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa
     1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1801 aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg
     1861 agccagtgag t
//
