LOCUS       Exported                1849 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    60..65
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    67..70
                     /label=U
                     /note="color: #ffff00"
     misc_feature    71..106
                     /label=conn U-V
                     /note="color: #c0c0c0"
     misc_feature    107..110
                     /label=V
                     /note="color: #ffff00"
     misc_feature    complement(112..117)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      192..221
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      243..830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      842..869
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(893..1708)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1709..1811)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      1812..1843
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcgtgga taatccaaca accccataac acccgccaca catacagttg cgagacgaaa
      121 acgaattcaa gcttgatatc attcaggacg agcctcagac tccagcgtaa ctggactgca
      181 atcaactcac tggctcacct tcacgggtgg gcctttcttc ggtagaaaat caaaggatct
      241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgag gtaactggct
      361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      781 ttgagcatcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      841 acgcagaaag gcccacccga aggtgagcca ggtgattaca tttgggccct cattagaaaa
      901 actcatcgag catcaagtga aactgcaatt tattcatatc aggattatca ataccatatt
      961 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     1021 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     1081 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     1141 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     1201 gctcgtcatc aaaatcactc gcaccaacca aaccgttatt cattcgtgat tgcgcctgag
     1261 cgagacgaaa tacgcgatcg ccgttaaaag gacaattaca aacaggaatc gaatgcaacc
     1321 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     1381 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag
     1441 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagcctga
     1501 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     1561 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     1621 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
     1681 aagacgtttc ccgttgaata tggctcattt tagcttcctt agctcctgaa aatctcgata
     1741 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1801 cgtgccgatc aagtcaaaag cctccggtcg gaggcttttg actttctgc
//
