LOCUS       Exported                1944 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    23..28
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    30..33
                     /label=W
                     /note="color: #ffff00"
     misc_feature    34..125
                     /label=conn W-Z
                     /note="color: #a6acb3"
     misc_feature    126..129
                     /label=Z
                     /note="color: #ffff00"
     misc_feature    complement(131..136)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      221..252
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        253..355
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             356..1171
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1195..1222
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1234..1821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1843..1872
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 actatcctaa cgtccacttc agcgtctcac gaacacatat tgatcctcgt cacaaccaca
       61 ccagcacacc tcaaatccca caccactccc acaattacca ttcactcaac aaactcacac
      121 atcccacgac gagacgcagg tggatgaaag cgtgtagacg aattctctag atatcgctca
      181 atactgacca tttaaatcat acctgacctc catagcagaa agtcaaaagc ctccgaccgg
      241 aggcttttga cttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
      301 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
      361 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
      421 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
      481 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
      541 caatgatgtt acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc
      601 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
      661 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
      721 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
      781 cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttgg
      841 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
      901 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
      961 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1021 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1081 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1141 gtttcacttg atgctcgatg agtttttcta atgagggccc aaatgtaatc acctggctca
     1201 ccttcgggtg ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1261 gcatcacaaa aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1681 atttggtatc tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga
     1741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1801 cgcagaaaaa aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg
     1861 tgaaggtgag ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg
     1921 aatgatatca agcttgaatt cgtt
//
