LOCUS       Exported                1931 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    9..14
                     /label=BsmBI
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    16..19
                     /label=A
                     /note="color: #ffff00"
     misc_feature    20..129
                     /label=Conn. BsaI-3
                     /note="color: #a6acb3"
     misc_feature    74..79
                     /label=BsaI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    81..84
                     /label=2
                     /note="color: #00ff00"
     misc_feature    130..133
                     /label=B
                     /note="color: #ffff00"
     misc_feature    complement(135..140)
                     /label=BsmBI
                     /note="color: #00ffff; direction: LEFT"
     terminator      208..239
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        240..342
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             343..1158
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1182..1209
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1221..1808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1830..1859
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 ctagtcaccg tctcgtagga gagcatggag aatgggagag aaagaaggga tgaaacaacg
       61 caacgaacga aaaggtctca atcccagctg ttaactagcg agtcagacga gatggataga
      121 tttagatgca tggcgagacg ctagacgaat tctctagata tcgctcaata ctgaccattt
      181 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt
      241 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      301 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg
      361 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      421 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
      481 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      541 gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      601 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg gaaaacagca
      661 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      721 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg cgatcgcgta
      781 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt
      841 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
      901 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
      961 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     1021 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     1081 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg
     1141 ctcgatgagt ttttctaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc
     1201 ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1261 cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1681 gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1801 gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca
     1861 gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc
     1921 ttgaattcgt t
//
