LOCUS       Exported                2503 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    68..73
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    75..78
                     /label=C
                     /note="color: #ffff00"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        478..753
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    769..772
                     /label=D
                     /note="color: #ffff00"
     misc_feature    complement(774..779)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      846..877
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        878..980
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             981..1796
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1820..1847
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1859..2446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      2468..2497
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
       61 ttcgttacgt ctcggactgg atctgatatc atcgtcgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
      481 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      541 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      601 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      661 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      721 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttggg accgagacga
      781 agacgaattc tctagatatc gctcaatact gaccatttaa atcatacctg acctccatag
      841 cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta agaggttcca
      901 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
      961 caggagctaa ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc
     1021 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     1081 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     1141 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact
     1201 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     1261 catggttact caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc
     1321 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     1381 ttcctgtttg taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat
     1441 cacgaatgaa taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc
     1501 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
     1561 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     1621 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     1681 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     1741 ataatcctga tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctaatgag
     1801 ggcccaaatg taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt
     1861 tccataggct ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc
     1921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2281 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2461 tttctaccga agaaaggccc acccgtgaag gtgagccagt gag
//
