LOCUS       Exported                2427 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    67..72
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    74..77
                     /label=Ha
                     /note="color: #ffff00"
     misc_feature    106..691
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 106 .. 679 / #a6acb3
                        2: 680 .. 682 / #a6acb3 / ATG
                        3: 683 .. 691 / #a6acb3"
     misc_feature    181..680
                     /label=IRES
                     /note="internal ribosome entry site"
                     /note="color: #cc0000; direction: RIGHT"
     CDS             680..682
                     /codon_start=1
                     /label=ATG
                     /note="color: #99cc00"
                     /translation="M"
     misc_feature    692..695
                     /label=I
                     /note="color: #ffff00"
     misc_feature    complement(697..702)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      769..800
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        801..903
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             904..1719
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1743..1770
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1782..2369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      2391..2420
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgttacgtc tcgatcccgc tcgataagct tgatatcgaa ttccgcccct ctccctcccc
      121 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
      181 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
      241 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
      301 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
      361 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
      421 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
      481 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
      541 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
      601 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
      661 tgaaaaacac gatgataata tggccacaac cgcgtcgaga cgaagacgaa ttctctagat
      721 atcgctcaat actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct
      781 ccgaccggag gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa
      841 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      901 aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg
      961 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1021 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1081 agcgttgcca atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg
     1141 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1201 gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1261 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1321 ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1381 ttggttggtg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1441 aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1501 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1561 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     1621 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     1681 aaattgcagt ttcacttgat gctcgatgag tttttctaat gagggcccaa atgtaatcac
     1741 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc
     1801 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta
     1861 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1921 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1981 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2041 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2101 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2161 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2221 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta
     2281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg
     2401 cccacccgtg aaggtgagcc agtgagt
//
