LOCUS Exported 2193 bp ds-DNA circular SYN 10-JUL-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS SnapGene DNA SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 2193) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Friday, Jul 21, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2193 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 65..70 /label=BsmBI /note="color: #a6acb3; direction: RIGHT" misc_feature 72..75 /label=N /note="color: #ffff00" promoter 76..405 /label=SV40 promoter /note="SV40 enhancer and early promoter" /note="color: #ffffff; direction: RIGHT" rep_origin 256..391 /label=SV40 ori /note="SV40 origin of replication" /note="color: #ffff00" misc_feature 456..459 /label=O /note="color: #ffff00" misc_feature complement(461..466) /label=BsmBI /note="color: #a6acb3; direction: LEFT" terminator 541..570 /label=T3Te terminator /note="phage T3 early transcription terminator" /note="color: #ff0000" rep_origin 592..1179 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" terminator 1191..1218 /label=T7Te terminator /note="phage T7 early transcription terminator" /note="color: #ff0000" CDS complement(1242..2057) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter complement(2058..2160) /label=cat promoter /note="promoter of the E. coli cat gene" /note="color: #ffffff; direction: LEFT" terminator 2161..2192 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" /note="color: #ff0000" ORIGIN 1 tctgctatgg aggtcaggta tgatttaaat ggtcagtatt gagcgatatc tagagaattc 61 gtcacgtctc gctacgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 121 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 181 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 241 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 301 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 361 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 421 tgtatatcca ttttcggatc tgatcaagag acagggcaac gagacgaaaa cgaattcaag 481 cttgatatca ttcaggacga gcctcagact ccagcgtaac tggactgcaa tcaactcact 541 ggctcacctt cacgggtggg cctttcttcg gtagaaaatc aaaggatctt cttgagatcc 601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 661 ttgtttgccg gatcaagagc taccaactct ttttccgagg taactggctt cagcagagcg 721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 1021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 1081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcatcga 1141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcagaaagg 1201 cccacccgaa ggtgagccag gtgattacat ttgggccctc attagaaaaa ctcatcgagc 1261 atcaagtgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc 1321 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg 1381 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca 1441 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc 1501 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca 1561 aaatcactcg caccaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat 1621 acgcgatcgc cgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac 1681 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat 1741 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa 1801 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagcctgac catctcatct 1861 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc 1921 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta 1981 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc 2041 cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat 2101 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca 2161 agtcaaaagc ctccggtcgg aggcttttga ctt //