LOCUS Exported 2415 bp ds-DNA circular SYN 10-JUL-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS SnapGene DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2415) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Friday, Jul 21, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2415 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 61..66 /label=BsmBI /note="color: #a6acb3; direction: RIGHT" misc_feature 68..71 /label=I /note="color: #ffff00" CDS 74..670 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" misc_feature 674..677 /label=J /note="color: #ffff00" misc_feature complement(679..684) /label=BsmBI /note="color: #a6acb3; direction: LEFT" terminator 759..788 /label=T3Te terminator /note="phage T3 early transcription terminator" /note="color: #ff0000" rep_origin 810..1397 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" terminator 1409..1436 /label=T7Te terminator /note="phage T7 early transcription terminator" /note="color: #ff0000" CDS complement(1460..2275) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter complement(2276..2378) /label=cat promoter /note="promoter of the E. coli cat gene" /note="color: #ffffff; direction: LEFT" terminator 2379..2410 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" /note="color: #ff0000" ORIGIN 1 ctatggaggt caggtatgat ttaaatggtc agtattgagc gatatctaga gaattcgtca 61 cgtctcggcg tccaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 121 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 181 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 241 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 301 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 361 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 421 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtg tcgcccgacc accagggcaa 481 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 541 cgccttcctg gaaacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 601 cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc 661 cggtgcctaa taatgctcga gacgaaaacg aattcaagct tgatatcatt caggacgagc 721 ctcagactcc agcgtaactg gactgcaatc aactcactgg ctcaccttca cgggtgggcc 781 tttcttcggt agaaaatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 901 ccaactcttt ttccgaggta actggcttca gcagagcgca gataccaaat actgttcttc 961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 1021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 1081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 1141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 1201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 1261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 1321 gtcctgtcgg gtttcgccac ctctgacttg agcatcgatt tttgtgatgc tcgtcagggg 1381 ggcggagcct atggaaaaac gccagcaacg cagaaaggcc cacccgaagg tgagccaggt 1441 gattacattt gggccctcat tagaaaaact catcgagcat caagtgaaac tgcaatttat 1501 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 1561 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 1621 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 1681 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc 1741 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca ccaaccaaac 1801 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgccg ttaaaaggac 1861 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1921 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag 1981 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 2041 taaattccgt cagccagttt agcctgacca tctcatctgt aacatcattg gcaacgctac 2101 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 2161 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 2221 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcattttag 2281 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt 2341 cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct ccggtcggag 2401 gcttttgact ttctg //