LOCUS       Exported                2042 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    60..65
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    67..70
                     /label=V
                     /note="color: #ffff00"
     polyA_signal    94..299
                     /label=PGK-polyA
                     /note="color: #333333"
     misc_feature    300..303
                     /label=W
                     /note="color: #ffff00"
     misc_feature    complement(305..310)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      385..414
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      436..1023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1035..1062
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(1086..1901)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1902..2004)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      2005..2036
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac
       61 gtctcggttg ccttgagcat ctgacttctg gctaaattga tgatctatta aacaataaag
      121 atgtccacat ggaagttttt tcctgtcata ctttgttaag aagggtgaga acagagtacc
      181 tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat
      241 gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca tagttggatc
      301 gaacgagacg aaaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg
      361 taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc ttcggtagaa
      421 aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      541 gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      601 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      661 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      721 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      781 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      841 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      901 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      961 cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1021 aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt acatttgggc
     1081 cctcattaga aaaactcatc gagcatcaag tgaaactgca atttattcat atcaggatta
     1141 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     1201 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     1261 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     1321 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     1381 ggccagccat tacgctcgtc atcaaaatca ctcgcaccaa ccaaaccgtt attcattcgt
     1441 gattgcgcct gagcgagacg aaatacgcga tcgccgttaa aaggacaatt acaaacagga
     1501 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     1561 ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat
     1621 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     1681 cagtttagcc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     1741 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     1801 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     1861 cgcggcctcg agcaagacgt ttcccgttga atatggctca ttttagcttc cttagctcct
     1921 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1981 ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg tcggaggctt ttgactttct
     2041 gc
//
