LOCUS       Exported                1847 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    65..70
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    72..75
                     /label=F
                     /note="color: #ffff00"
     misc_feature    76..83
                     /label=Kozak
                     /note="color: #99cc00"
     CDS             84..86
                     /codon_start=1
                     /label=ATG
                     /note="color: #ff0000"
                     /translation="M"
     CDS             87..107
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #ff9900"
                     /translation="PKKKRKV"
     misc_feature    110..113
                     /label=G
                     /note="color: #ffff00"
     misc_feature    complement(115..120)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      187..218
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        219..321
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             322..1137
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1161..1188
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1200..1787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1809..1838
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa gcttgaattc
       61 gttacgtctc gccagccgcc accatgccta agaagaaacg gaaagtgggc agccgagacg
      121 aagacgaatt ctctagatat cgctcaatac tgaccattta aatcatacct gacctccata
      181 gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc
      241 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      301 tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcgaggccg
      361 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      421 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
      481 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt caggctaaac
      541 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
      601 gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat
      661 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
      721 attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc tcaggcgcaa
      781 tcacgaatga ataacggttt ggttggtgcg agtgattttg atgacgagcg taatggctgg
      841 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
      901 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
      961 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1021 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1081 gataatcctg atatgaataa attgcagttt cacttgatgc tcgatgagtt tttctaatga
     1141 gggcccaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt
     1201 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg
     1261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1381 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1801 ttttctaccg aagaaaggcc cacccgtgaa ggtgagccag tgagttg
//
