LOCUS       Exported                2140 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    SnapGene DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    64..69
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    71..74
                     /label=U
                     /note="color: #ffff00"
     CDS             75..395
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP (DmrA)
                     /note="color: #993366"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    402..405
                     /label=V
                     /note="color: #ffff00"
     misc_feature    complement(407..412)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      487..516
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      538..1125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1137..1164
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(1188..2003)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(2004..2106)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      2107..2138
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 ctgctatgga ggtcaggtat gatttaaatg gtcagtattg agcgatatct agagaattcg
       61 tcacgtctcg tggaggagtg caggtggaaa ccatctcccc aggcgacggg cgcaccttcc
      121 ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat
      181 ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga
      241 tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta
      301 tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca
      361 ctctcgtctt cgatgtggag cttctaaaac tggaataata agttgcgaga cgaaaacgaa
      421 ttcaagcttg atatcattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
      481 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
      541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
      661 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      841 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      901 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      961 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1081 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
     1141 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta gaaaaactca
     1201 tcgagcatca agtgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     1261 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     1321 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     1381 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     1441 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     1501 tcatcaaaat cactcgcacc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     1561 cgaaatacgc gatcgccgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     1621 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     1681 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     1741 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag cctgaccatc
     1801 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     1861 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     1921 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac
     1981 gtttcccgtt gaatatggct cattttagct tccttagctc ctgaaaatct cgataactca
     2041 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     2101 cgatcaagtc aaaagcctcc ggtcggaggc ttttgacttt
//
