LOCUS       Exported                2355 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HC_Kan_CMVp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    67..72
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    74..77
                     /label=R
                     /note="color: #ffff00"
     enhancer        79..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        382..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    621..624
                     /label=S
                     /note="color: #ffff00"
     misc_feature    complement(626..631)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      697..728
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        729..831
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             832..1647
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1671..1698
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1710..2297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      2319..2348
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgttacgtc tcgcggtcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctaattc gtcgactgga
      601 tccggtaccg aggagatctg gtgccgagac gaaacgaatt ctctagatat cgctcaatac
      661 tgaccattta aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc
      721 ttttgacttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      781 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat
      841 attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta
      901 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
      961 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1021 gatgttacag atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc
     1081 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg
     1141 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1201 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc
     1261 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg
     1321 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1381 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1441 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1501 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1561 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1621 cacttgatgc tcgatgagtt tttctaatga gggcccaaat gtaatcacct ggctcacctt
     1681 cgggtgggcc tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1741 cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2161 ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg
     2221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2281 gaaaaaaagg atctcaagaa gatcctttga ttttctaccg aagaaaggcc cacccgtgaa
     2341 ggtgagccag tgagt
//
