LOCUS       Exported                2423 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HC_Kan_PuroR-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    70..75
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    77..80
                     /label=O
                     /note="color: #ffff00"
     misc_feature    81..88
                     /label=KozaK
                     /note="color: #99cc00"
     CDS             89..91
                     /codon_start=1
                     /label=ATG
                     /note="color: #ff0000"
                     /translation="M"
     CDS             92..688
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     misc_feature    692..695
                     /label=P
                     /note="color: #ffff00"
     misc_feature    complement(697..702)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      768..799
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        800..902
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             903..1718
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      1742..1769
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(1781..2368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      2390..2419
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat atcaagcttg
       61 aattcgttac gtctcggcaa ccgccaccat gaccgagtac aagcccacgg tgcgcctcgc
      121 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
      181 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
      241 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
      301 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
      361 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
      421 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtgtc
      481 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
      541 cgagcgcgcc ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc tccccttcta
      601 cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg
      661 gtgcatgacc cgcaagcccg gtgcctaata accctcgaga cgaaacgaat tctctagata
      721 tcgctcaata ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc
      781 cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa
      841 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
      901 aaatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
      961 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1021 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1081 gcgttgccaa tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc
     1141 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1201 cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     1261 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     1321 cttttaacgg cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     1381 tggttggtgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     1441 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     1501 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     1561 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     1621 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     1681 aattgcagtt tcacttgatg ctcgatgagt ttttctaatg agggcccaaa tgtaatcacc
     1741 tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc
     1801 ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat
     1861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2221 gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag
     2281 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2341 gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc gaagaaaggc
     2401 ccacccgtga aggtgagcca gtg
//
