LOCUS       Exported                2101 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    HC_Kan_DmrC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    63..68
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    70..73
                     /label=I
                     /note="color: #ffff00"
     CDS             76..354
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB* (DmrC)
                     /note="binds synthetic ligands such as AP21967"
                     /note="color: #993366"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     misc_feature    362..365
                     /label=J
                     /note="color: #ffff00"
     misc_feature    complement(367..372)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      447..476
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      498..1085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      1097..1124
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     CDS             complement(1148..1963)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1964..2066)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: LEFT"
     terminator      2067..2098
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
ORIGIN
        1 tgctatggag gtcaggtatg atttaaatgg tcagtattga gcgatatcta gagaattcgt
       61 cacgtctcgg cgtccatcct ctggcatgag atgtggcatg aaggcctgga agaggcatct
      121 cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat
      181 gctatgatgg aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt
      241 cgagatttaa tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag
      301 gacctcctcc aagcctggga cctctattat catgtgttcc gacgaatctc aaagtaataa
      361 ctgctcgaga cgaaaacgaa ttcaagcttg atatcattca ggacgagcct cagactccag
      421 cgtaactgga ctgcaatcaa ctcactggct caccttcacg ggtgggcctt tcttcggtag
      481 aaaatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      541 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      601 ccgaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      661 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      721 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      781 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      841 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      901 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      961 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1021 ttcgccacct ctgacttgag catcgatttt tgtgatgctc gtcagggggg cggagcctat
     1081 ggaaaaacgc cagcaacgca gaaaggccca cccgaaggtg agccaggtga ttacatttgg
     1141 gccctcatta gaaaaactca tcgagcatca agtgaaactg caatttattc atatcaggat
     1201 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     1261 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     1321 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     1381 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     1441 caggccagcc attacgctcg tcatcaaaat cactcgcacc aaccaaaccg ttattcattc
     1501 gtgattgcgc ctgagcgaga cgaaatacgc gatcgccgtt aaaaggacaa ttacaaacag
     1561 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     1621 caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc
     1681 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     1741 gccagtttag cctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     1801 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     1861 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     1921 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cattttagct tccttagctc
     1981 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2041 agttggaacc tcttacgtgc cgatcaagtc aaaagcctcc ggtcggaggc ttttgacttt
     2101 c
//
