LOCUS       Exported                2735 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    66..71
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    73..76
                     /label=J
                     /note="color: #ffff00"
     misc_feature    87..675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     LTR             747..980
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    999..1002
                     /label=K
                     /note="color: #ffff00"
     misc_feature    complement(1004..1009)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      1076..1107
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        1108..1210
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1211..2026
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2050..2077
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(2089..2676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      2698..2727
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
       61 cgttacgtct cgtgctttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
      121 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
      181 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
      241 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
      301 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
      361 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
      421 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
      481 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
      541 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
      601 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
      661 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
      721 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
      781 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      961 agtgtggaaa atctctagca gggcccgttt aaacccgcgg tacgagacga agacgaattc
     1021 tctagatatc gctcaatact gaccatttaa atcatacctg acctccatag cagaaagtca
     1081 aaagcctccg accggaggct tttgacttga tcggcacgta agaggttcca actttcacca
     1141 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1201 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
     1261 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1321 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1381 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga
     1441 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1501 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1561 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1621 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1681 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1741 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
     1801 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1861 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1921 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1981 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctaatgag ggcccaaatg
     2041 taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt tccataggct
     2101 ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc gaaacccgac
     2161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct cggaaaaaga
     2581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat tttctaccga
     2701 agaaaggccc acccgtgaag gtgagccagt gagtt
//
