LOCUS       Exported                3781 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
VERSION     .
KEYWORDS    pSMART HCKan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    70..75
                     /label=BsmBI
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    77..80
                     /label=A
                     /note="color: #ffff00"
     promoter        82..308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             309..489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    309..489
                     /label=5'LTR (R and U5 portions; U3 was replaced by CMVp)
                     /note="color: #a6acb3"
     misc_feature    536..661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    645..1009
                     /label=gag
                     /note="color: #ffcc99"
     misc_feature    1154..1396
                     /label=RRE
                     /note="color: #ffcc99"
     misc_feature    1914..2031
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2049..2052
                     /label=B
                     /note="color: #ffff00"
     misc_feature    complement(2054..2059)
                     /label=BsmBI
                     /note="color: #a6acb3; direction: LEFT"
     terminator      2126..2157
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ff0000"
     promoter        2158..2260
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2261..3076
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      3100..3127
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
                     /note="color: #ff0000"
     rep_origin      complement(3139..3726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     terminator      3748..3777
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
                     /note="color: #ff0000"
ORIGIN
        1 agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat atcaagcttg
       61 aattcgttac gtctcgtagg atgtagtctt atgcaatact cttgtagtct tgcaacatgg
      121 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
      181 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
      241 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
      301 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      481 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
      901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
      961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1021 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1081 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1141 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1201 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1261 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1321 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1381 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1441 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1501 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1561 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1621 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1681 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1741 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1801 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1861 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     1921 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1981 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2041 tacagggaat ggcgagacga agacgaattc tctagatatc gctcaatact gaccatttaa
     2101 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
     2161 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     2221 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
     2281 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2341 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     2401 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2461 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2521 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
     2581 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2641 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
     2701 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
     2761 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     2821 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2881 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2941 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3001 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
     3061 cgatgagttt ttctaatgag ggcccaaatg taatcacctg gctcaccttc gggtgggcct
     3121 ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3181 atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3541 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3601 tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3721 tctcaagaag atcctttgat tttctaccga agaaaggccc acccgtgaag gtgagccagt
     3781 g
//
