LOCUS       Exported               15257 bp ds-DNA     circular SYN 27-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAEL007097-Cas9.sbd
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2017 from SnapGene Viewer 4.0.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15257)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    854..1714)
                     /label=AmpR
                     /note="Unknown feature type:ORF pUC bla Gene Amp Resist."
     old_sequence    2319..3026)
                     /label=flanking sequence
     misc_binding    complement(3027..4024))
                     /label=piggyBacR
     misc_binding    4073..4357)
                     /label=attB site
                     /note="attB site"
     primer_bind     4073..4096)
                     /label=Primer 41
     misc_feature    complement(4079..4102))
                     /note="attBseqR Primer 191"
     primer_bind     complement(4340..4357))
                     /label=Primer 43
     misc_feature    4366..6865)
                     /note="AAEL007097promoter + 5'UTR for cloning"
     primer_bind     4366..4384)
                     /label=Primer 55
     misc_feature    6744..6865)
                     /note="5'UTR"
     primer_bind     complement(6840..6865))
                     /label=Primer 62
     misc_feature    6874..6993)
                     /label=NLS
     primer_bind     6874..6898)
                     /label=Primer 39
     misc_feature    6994..11094)
                     /label=hSpCas9
     primer_bind     complement(8730..8740))
                     /label=Primer 39
     misc_feature    11095..11142)
                     /label=NLS(1)
     primer_bind     complement(11121..11142))
                     /label=Primer 40
     misc_feature    11143..11196)
                     /label=T2A
                     /note="T2A (ribosomal skipping element from insect Thosea 
                     asigna virus (TaV, T2A)"
     misc_feature    complement(11167..11170))
                     /label=Untitled
     misc_feature    11191..11196)
                     /note="Glycine-Proline Clevage site"
     misc_feature    11197..11916)
                     /note="GFP"
     primer_bind     11197..11217)
                     /label=Primer 46
     primer_bind     complement(11893..11916))
                     /label=Primer 54
     misc_feature    11925..12601)
                     /note="p10-3'-UTR-Rubin"
     primer_bind     11925..11956)
                     /label=Primer 52
     primer_bind     complement(12582..12601))
                     /label=Primer 50
     misc_feature    12610..13150)
                     /note="Opie2 promoter"
     primer_bind     12610..12630)
                     /label=Primer 8
     primer_bind     complement(13045..13071))
                     /label=Primer 9
     primer_bind     complement(13097..13115))
                     /label=Primer 11
     primer_bind     complement(13131..13150))
                     /label=Primer 12
     misc_feature    13151..13831)
                     /note="Dsred"
     misc_feature    13478..13486)
                     /note="pt mutation"
     misc_feature    13832..14072)
                     /note="Sv40"
     primer_bind     13832..13858)
                     /label=Primer 16
     primer_bind     complement(14043..14072))
                     /label=Primer 19
     misc_binding    14084..14784)
                     /label=piggyBacL
     old_sequence    14785..14913)
                     /label=flanking seq
     misc_feature    complement(14914..15257))
                     /label=Untitled
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccggggtcct aggtcgacga
     4081 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     4141 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
     4201 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
     4261 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
     4321 tcagcgggtt ctcgacggtc acggcgggca tgtcgacgcg gccgcacgtt ttgtgcgctc
     4381 cgggaaaagt cacgttgttg ataaatgaat atcctggatc cacgcacata caaaacatct
     4441 tccttgataa gagctattaa ccctcttttt cccacgactt tgattgacaa tttcttgaag
     4501 tgcttgagca aataatgttg caaatggtct acaccaggcc tgcccaacct ttttgaaccg
     4561 cgggccaaat cgcagaaata attttgtgtc gcgggccaca tttttaaacg cacaaatatt
     4621 tccataaagt ctggcaaaag cctgtaaatg aagttcaaaa aacctgacaa aaatcagtga
     4681 atggagtcaa tttcatttga agaaatcgaa aaaagaaaag cttaacagca attaagcatt
     4741 ctgctggaca ttgtgaaaat cccttacaaa attatatgat aatatagtcc aaaatattta
     4801 gaatagaatc tatcctagaa tgatactaga atttttttca aacgtactcc gaattttgtc
     4861 agtatccgac ttaaaattct tagcatgaac cggaatgata aactctgatc acgcttttac
     4921 gaaaatcctg gtaggaagtg gtaaagaaaa tcacatttct ttaatcgcag tactcagttg
     4981 agataaaatg tcatgtcaaa tggggctctc tgaattattt tttttttacc catccactca
     5041 aggaattgct ttatttttct cgagcgaaac agcaaactca gttttatcca cgaaagtcgt
     5101 gtccagaatt cttaaaaaat cctgttcaag attttgtaaa atgcccgcct tttctataat
     5161 cctatcaagg ataaatgtcg aatcaatgaa tgaatgtaca gtgatacctc catgagtcga
     5221 tgttccatga ctcgatatcg actcatggaa ccatactaaa aacaaaattt catggttact
     5281 attactatac atactaaaaa caaaattaca tggttaggga ccatcatccc tataagcagg
     5341 tttgcaaagg attgctgttc catgactcga tatttccatg agtcgatggt cccttcaata
     5401 tcgactcatg gaggtttcac tgtagatgga agtcatggta gaaatattgt tgaaacactc
     5461 cgaattcgag attttgaatt tgaatcatcc atacaattcg atatctaaga aggttaaaaa
     5521 acaaaacaat ttaaataatt actaagctag aatatgatat tgaaactaaa ttgaaaattt
     5581 aatagtcttg gtatggtaac tttgggttgg tagaatattc agcgggcaaa atataagaag
     5641 aatcttcgaa ccctccgcgg gccgcaaaaa atggagtcgc gggccggatg cggcccgcgg
     5701 gccgtacgtt gggcagccct ggtctacact tttcgaaatc ggtttatttt tttgtttgat
     5761 tgtttatcaa tagttttact attgaaactc ttagtttact tttggattaa tctgctgagt
     5821 atagaatcat attgtagtaa ctatgtattg catagcattc atctggttct caagtttgtc
     5881 gaaaacgaat ggagctctgg aaccaccatg gaaaagagag ggttaaattg attgtcctca
     5941 tttattcagt attgaagcat attggcatca attcgctcac agacggttat tcgctcactt
     6001 ctcaactaca attcaaatac aattatgatc gatgtatgcg atcgatcgat acgttgcctc
     6061 ttatagaata tcattatcat catcgttttc aattgaacga taactcacaa acccgtaatc
     6121 atcaactcag ataatgatca agaacccatt tgggcagttt gtgcaagtga gctaaacaga
     6181 tgattcatgg tactcagtaa ataatggaaa tgatttcgat atcaccatat agcgtgaata
     6241 gcaatccttt ctaatagttg ccactagttt taacattcag ttttaaagtc atgttcagta
     6301 gcataacatt ttgtagcaga attgaaattt tatgagcata atttacaatt cataattgca
     6361 atcctgttag ccaatttgag tgacttaaaa ctcaatggct gtgtatgatc aatcttgtgt
     6421 agcaaattta gagtcattag attacaaaca atattgatac aaacttatga tttttttcat
     6481 caatattttt atcagaattt tatcaggtat taaaatttca ctcagttcag tgtccacata
     6541 tttccgcata tgaacgatat tgccacgaaa accgatcgaa agaataagat agcttatcag
     6601 tgagatactg ttattcgaaa caatgctctt ctcggtagaa acaatcacat ctgtcgcttt
     6661 cctatccgca acatgggcta accatgcaga tctcctttat ctctctcgac tataaatagg
     6721 gccttgaggt cactcaccgc ttcaaaattg aactactatc cagagaagaa agttaagttt
     6781 tatattcaag tgacaaagtg acagaaaact aagtgtattc aatagttgtt ttaaataagt
     6841 gtgttaataa gtgtccaaaa tccaagttta aacatggact ataaggacca cgacggagac
     6901 tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag
     6961 cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac
     7021 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     7081 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
     7141 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
     7201 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
     7261 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
     7321 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     7381 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
     7441 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
     7501 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     7561 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
     7621 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
     7681 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
     7741 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     7801 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     7861 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     7921 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     7981 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     8041 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     8101 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     8161 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     8221 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     8281 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     8341 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     8401 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     8461 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     8521 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     8581 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     8641 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     8701 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     8761 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     8821 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     8881 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     8941 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     9001 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     9061 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     9121 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     9181 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     9241 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     9301 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     9361 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     9421 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     9481 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac
     9541 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     9601 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     9661 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     9721 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     9781 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     9841 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     9901 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     9961 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
    10021 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
    10081 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
    10141 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
    10201 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
    10261 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
    10321 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
    10381 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
    10441 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
    10501 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
    10561 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
    10621 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
    10681 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
    10741 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
    10801 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
    10861 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
    10921 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
    10981 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
    11041 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaaaagg
    11101 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa aggagggcag aggaagtctt
    11161 ctaacatgcg gtgacgtgga ggagaatccc ggccctatgg tgagcaaggg cgaggagctg
    11221 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
    11281 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
    11341 tgcaccacca gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
    11401 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
    11461 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
    11521 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
    11581 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
    11641 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
    11701 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
    11761 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
    11821 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
    11881 gggatcactc tcggcatgga cgagctgtac aagtaattaa ttaactagaa tgaatcgttt
    11941 ttaaaataac aaatcaattg ttttataata ttcgtacgat tctttgatta tgtaataaaa
    12001 tgtgatcatt aggaagatta cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa
    12061 tgctgtaaac gccacaattg tgtttgttgc aaataaaccc atgattattt gattaaaatt
    12121 gttgttttct ttgttcatag acaatagtgt gttttgccta aacgtgtact gcataaactc
    12181 catgcgagtg tatagcgagc tagtggctaa cgcttgcccc accaaagtag attcgtcaaa
    12241 atcctcaatt tcatcaccct cctccaagtt taacatttgg ccgtcggaat taacttctaa
    12301 agatgccaca taatctaata aatgaaatag agattcaaac gtggcgtcat cgtccgtttc
    12361 gaccatttcc gaaaagaact cgggcataaa ctctatgatt tctctggacg tggtgttgtc
    12421 gaaactctca aagtacgcag tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg
    12481 aaacatgttg ttgtaaccga acgggtccca tagcgccaaa accaaatctg ccagcgtcaa
    12541 tagaatgagc acgatgccga caatggagct ggcttggata gcgattcgag ttaacggccg
    12601 gggcgcgccc atactcggtg gcctccccac caccaacttt tttgcactgc aaaaaaacac
    12661 gcttttgcac gcgggcccat acatagtaca aactctacgt ttcgtagact attttacata
    12721 aatagtctac accgttgtat acgctccaaa tacactacca cacattgaac ctttttgcag
    12781 tgcaaaaaag tacgtgtcgg cagtcacgta ggccggcctt atcgggtcgc gtcctgtcac
    12841 gtacgaatca cattatcgga ccggacgagt gttgtcttat cgtgacagga cgccagcttc
    12901 ctgtgttgct aaccgcagcc ggacgcaact ccttatcgga acaggacgcg cctccatatc
    12961 agccgcgcgt tatctcatgc gcgtgaccgg acacgaggcg cccgtcccgc ttatcgcgcc
    13021 tataaataca gcccgcaacg atctggtaaa cacagttgaa cagcatctgt tacagcgaca
    13081 caacatgagc cggtccaaca acgccaacgc gcccacgcca tccaaccgcc gccgcaacct
    13141 gtctctggtg atggtgcgct cctccaagaa cgtcatcaag gagttcatgc gcttcaaggt
    13201 gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
    13261 cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt
    13321 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
    13381 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
    13441 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
    13501 ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat
    13561 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
    13621 gctgaagggc gagatccaca aggccctgaa gctgaaggac ggcggccact acctggtgga
    13681 gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
    13741 ctccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg
    13801 caccgagggc cgccaccacc tgttcctgta ggactctaga tcataatcag ccataccaca
    13861 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    13921 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    13981 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    14041 ttgtccaaac tcatcaatgt atcttaaagc ttctaggccg gccgatctcg gatctgacaa
    14101 tgttcagtgc agagactcgg ctacgcctcg tggactttga agttgaccaa caatgtttat
    14161 tcttacctct aatagtcctc tgtggcaagg tcaagattct gttagaagcc aatgaagaac
    14221 ctggttgttc aataacattt tgttcgtcta atatttcact accgcttgac gttggctgca
    14281 cttcatgtac ctcatctata aacgcttctt ctgtatcgct ctggacgtca tcttcactta
    14341 cgtgatctga tatttcactg tcagaatcct caccaacaag ctcgtcatcg ctttgcagaa
    14401 gagcagagag gatatgctca tcgtctaaag aactacccat tttattatat attagtcacg
    14461 atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
    14521 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
    14581 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
    14641 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
    14701 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
    14761 ttctagggtt aaaaaagatt tgcgctttac tcgacctaaa ctttaaacac gtcatagaat
    14821 cttcgtttga caaaaaccac attgtggcca agctgtgtga cgcgacgcgc gctaaagaat
    14881 ggcaaaccaa gtcgcgcgag cgtcgactct agaggatccc cgggtaccga gctcgaattc
    14941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    15001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    15061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    15121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    15181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    15241 aaaggcggta atacggt
//
