LOCUS       FKBP-beta2(616-951)-GFP_ÆCBM.xdna    6073 bp      DNA     circular   28/07/2017
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pEGFP-N1 [4676  nt] : (AgeI[666] / XhoI[613]) 
            to FKBP-Beta2-mCherry [1416  nt] : (XhoI[613] / AgeI[2033])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     rep_origin      5349..5977
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      2999..3439
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3972..4763
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        3738..3934
                     /label=SV40 early promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2022..2735
                     /label=EGFP
                     /SerialCloner_Color=&h32CD32
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        3506..3534
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3785..3862
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     618..1057
                     /label=insert from FKBP-Beta2-mCherry [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from FKBP-Beta2-mCherry [1416  nt] : (XhoI[613] / AgeI[2033]) \ 
                     1058..2006
                     /label=insert from FKBP-Beta2-mCherry [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from FKBP-Beta2-mCherry [1416  nt] : (XhoI[613] / AgeI[2033]) \ 
     misc_feature    1012..1012
                     /label=beta2(616-951) [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1024..1057
                     /label=beta2(616-951) [3'] [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1058..2003
                     /label=beta2(616-951) [3'] [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 
      601 CCGGACTCAG ATCtcgagag atcgatgtga atgggggccc tgAACCCGCC AAGAACCTGT 
      661 ACACCATGGG AGTGCAGGTG GAAACCATCT CCCCAGGAGA CGGGCGCACC TTCCCCAAGC 
      721 GCGGCCAGAC CTGCGTGGTG CACTACACCG GGATGCTTGA AGATGGAAAG AAATTTGATT 
      781 CCTCCCGGGA CAGAAACAAG CCCTTTAAGT TTATGCTAGG CAAGCAGGAG GTGATCCGAG 
      841 GCTGGGAAGA AGGGGTTGCC CAGATGAGTG TGGGTCAGAG AGCCAAACTG ACTATATCTC 
      901 CAGATTATGC CTATGGTGCC ACTGGGCACC CAGGCATCAT CCCACCACAT GCCACTCTcg 
      961 tcttcgatgt ggagcttcta aaactggaac ggtacCGCGG GCCCGGGATC CCCgccaccA 
     1021 TGgaacagcc tcaggttatc ccctctcaag gtgatctcgg tcccccagtc aatgtgccac 
     1081 aggtgtcctc catgcagatg ggagcagtgg atctcctagg aggaggacta gatagtctgc 
     1141 ttggcagtga ccttggcggg ggcattggag gaagtccggc agtgggacaa tccttcatcc 
     1201 catcatcggt ccctgcaacc tttgctcctt cacctacacc tgctgtggtc agcagtggac 
     1261 tgaatgacct gtttgaactc tccacaggga taggcatggc acctggtgga tatgtggctc 
     1321 ctaaggctgt ctggctacct gcagtaaagg ctaaaggctt ggagatttcc ggaacattta 
     1381 ctcaccgcca agggcacatc tatatggaaa tgaacttcac caataaagct ctgcagcaca 
     1441 tgacagattt tgcaatccag tttaacaaaa atagctttgg tgtcatcccc agcactcctc 
     1501 tggccatcca tacaccactg atgccaaacc agagcattga tgtctccctg cctctcaata 
     1561 ccttgggccc agtcatgaag atggaacctc tgaataacct ccaggtggct gtgaaaaaca 
     1621 atatcgatgt cttctacttc agctgcctca tcccactcaa tgtgcttttt gtagaagatg 
     1681 gcaaaatgga gcgccaggtc ttccttgcaa catggaagga tattcccaat gaaaatgaac 
     1741 ttcagtttca gattaaggaa tgtcatttaa atgctgacac tgtttccagc aagttgcaaa 
     1801 acaacaatgt ttatactatt gccaagagga atgtggaagg gcaggacatg ctgtaccaat 
     1861 ccctgaagct cactaatggc atttggattt tggccgaact acgtatccag ccaggaaacc 
     1921 ccaattacac gctgtcactg aagtgtagag ctcctgaagt ctctcaatac atctatcagg 
     1981 tctacgacag cattttgaaa aacttAccgg TCGCCACCAT GGTGAGCAAG GGCGAGGAGC 
     2041 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT 
     2101 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA 
     2161 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG 
     2221 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG 
     2281 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA 
     2341 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG 
     2401 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA 
     2461 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA 
     2521 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC 
     2581 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC 
     2641 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG 
     2701 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGCGAC TCTAGATCAT 
     2761 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC 
     2821 CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA 
     2881 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 
     2941 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGGCGTAA ATTGTAAGCG 
     3001 TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT 
     3061 AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG 
     3121 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC 
     3181 GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT 
     3241 TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG 
     3301 CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG 
     3361 GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC 
     3421 TTAATGCGCC GCTACAGGGC GCGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 
     3481 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 
     3541 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG 
     3601 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 
     3661 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 
     3721 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC 
     3781 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT 
     3841 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG 
     3901 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT 
     3961 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 
     4021 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 
     4081 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 
     4141 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 
     4201 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 
     4261 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 
     4321 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 
     4381 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 
     4441 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA 
     4501 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 
     4561 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 
     4621 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 
     4681 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 
     4741 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC 
     4801 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 
     4861 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 
     4921 GTTCTTCGCC CACCCTAGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG 
     4981 AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT 
     5041 TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA 
     5101 TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA 
     5161 AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCCT CAGGTTACTC 
     5221 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 
     5281 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 
     5341 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 
     5401 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 
     5461 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 
     5521 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 
     5581 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 
     5641 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 
     5701 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 
     5761 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 
     5821 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 
     5881 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 
     5941 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 
     6001 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 
     6061 TACCGCCATG CAT
//