LOCUS       PDZb1-beta2(616-951)-mCherry.xdna    6292 bp      DNA     circular   28/07/2017
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : FKBP-Beta2-mCherry [5693  nt] : (BamHI[1006] /
             XhoI[613]) to PDZb-b2-GFP [591  nt] : (XhoI[613] / BamHI[12
            08])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2248..2958
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     618..1208
                     /label=insert from PDZb-b2-GFP
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PDZb-b2-GFP [591  nt] : (XhoI[613] / BamHI[1208]) \ 
     misc_feature    1226..2232
                     /label=beta2(616-951) [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1225..2232
                     /label=Beta2
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="EQPQVIPSQGDLLGDLLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVVSSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNN \ 
                     621..1004
                     /label=insert from PCR(pDS221 insert-ePDZb1ÐmCherry (Budding Yeast) .xdna) [*] [3'] [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(pDS221 insert-ePDZb1ÐmCherry (Budding Yeast) .xdna) [591  nt] : (XhoI[7] / BamHI[602]) \ 
                     1005..1005
                     /label=insert from PCR(pDS221 insert-ePDZb1ÐmCherry (Budding Yeast) .xdna) [*] [3'] [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(pDS221 insert-ePDZb1ÐmCherry (Budding Yeast) .xdna) [591  nt] : (XhoI[7] / BamHI[602]) \ 
                     1024..1208
                     /label=insert from PCR(pDS221 insert-ePDZb1ÐmCherry (Budding Yeast) .xdna) [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(pDS221 insert-ePDZb1ÐmCherry (Budding Yeast) .xdna) [591  nt] : (XhoI[7] / BamHI[602]) \ 
     misc_feature    623..1004
                     /label=Lovpep [*]
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1024..1054
                     /label=Lovpep [*] [3']
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    621..1004
                     /label=Lovpep [*]
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1024..1052
                     /label=Lovpep [*] [3']
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1024..1053
                     /label=Lovpep [*] [3']
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    622..1004
                     /label=Lovpep [*]
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1005..1005
                     /label=Lovpep [*] [5']
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1024..1050
                     /label=Lovpep [*] [3']
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    625..1209
                     /label=PDZ [*]
                     /SerialCloner_Color=&h00FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      5568..6196
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      3218..3658
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4191..4982
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        3957..4153
                     /label=SV40 early promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        3725..3753
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4004..4081
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 
      601 CCGGACTCAG ATCtcgaggc caccatgcca gaacttggat ttagCATATC AGGTGGTGTC 
      661 GGGGGTAGAG GAAACCCATT CAGACCTGAT GATGATGGTA TATTTGTAAC AAGGGTACAA 
      721 CCTGAAGGAC CAGCATCAAA ATTACTGCAG CCAGGTGATA AAATTATTCA GGCTAATGGC 
      781 TACAGTTTTA TAAATATTGA ACATGGACAA GCAGTGTCCT TGCTAAAAAC TTTCCAGAAT 
      841 ACAGTTGAAC TCATCATTGT ACGAGAAGTT GGTAACGGTG CAAAACAAGA GATTCGAGTG 
      901 AGGGTTGAAA AAGACGGTGG TTCTGGAGGC GTTTCTTCTG TTCCGACCAA TCTGGAAGTT 
      961 GTTGCTGCGA CCCCGACTAG CCTGCTGATC AGCTGGGATG CGTATAGGGA GCTTCCGGTT 
     1021 AGTTATTACC GTATCACGTA CGGTGAAACC GGTGGTAACT CCCCGGTTCA GGAATTCACT 
     1081 GTACCTGGTT CCAAGTCTAC TGCTACCATC AGCGGCCTGA AACCGGGTGT CGACTATACC 
     1141 ATCACTGTAT ACGCACATTA CAACTACCAT TACTACTCTA GCCCAATctc gattaactac 
     1201 cgtaccagga tcCCCgccac cATGgaacag cctcaggtta tcccctctca aggtgatctt 
     1261 ctaggggatc ttttaaacct tgacctcggt cccccagtca atgtgccaca ggtgtcctcc 
     1321 atgcagatgg gagcagtgga tctcctagga ggaggactag atagtctgct tggcagtgac 
     1381 cttggcgggg gcattggagg aagtccggca gtgggacaat ccttcatccc atcatcggtc 
     1441 cctgcaacct ttgctccttc acctacacct gctgtggtca gcagtggact gaatgacctg 
     1501 tttgaactct ccacagggat aggcatggca cctggtggat atgtggctcc taaggctgtc 
     1561 tggctacctg cagtaaaggc taaaggcttg gagatttccg gaacatttac tcaccgccaa 
     1621 gggcacatct atatggaaat gaacttcacc aataaagctc tgcagcacat gacagatttt 
     1681 gcaatccagt ttaacaaaaa tagctttggt gtcatcccca gcactcctct ggccatccat 
     1741 acaccactga tgccaaacca gagcattgat gtctccctgc ctctcaatac cttgggccca 
     1801 gtcatgaaga tggaacctct gaataacctc caggtggctg tgaaaaacaa tatcgatgtc 
     1861 ttctacttca gctgcctcat cccactcaat gtgctttttg tagaagatgg caaaatggag 
     1921 cgccaggtct tccttgcaac atggaaggat attcccaatg aaaatgaact tcagtttcag 
     1981 attaaggaat gtcatttaaa tgctgacact gtttccagca agttgcaaaa caacaatgtt 
     2041 tatactattg ccaagaggaa tgtggaaggg caggacatgc tgtaccaatc cctgaagctc 
     2101 actaatggca tttggatttt ggccgaacta cgtatccagc caggaaaccc caattacacg 
     2161 ctgtcactga agtgtagagc tcctgaagtc tctcaataca tctatcaggt ctacgacagc 
     2221 attttgaaaa acttACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC 
     2281 ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG 
     2341 TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG 
     2401 AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG 
     2461 TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC 
     2521 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC 
     2581 GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC 
     2641 ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC 
     2701 TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG 
     2761 CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC 
     2821 GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG 
     2881 GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG 
     2941 GACGAGCTGT ACAAGTAAGC GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT 
     3001 AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT 
     3061 GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 
     3121 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 
     3181 CAAACTCATC AATGTATCTT AAGGCGTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG 
     3241 CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC 
     3301 CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA 
     3361 GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG 
     3421 ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG 
     3481 CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA 
     3541 ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG 
     3601 TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG 
     3661 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA 
     3721 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 
     3781 GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG 
     3841 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 
     3901 AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 
     3961 TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC 
     4021 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC 
     4081 CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 
     4141 AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG 
     4201 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG 
     4261 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 
     4321 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG 
     4381 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA 
     4441 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT 
     4501 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA 
     4561 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 
     4621 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC 
     4681 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG 
     4741 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG 
     4801 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA 
     4861 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 
     4921 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT 
     4981 GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 
     5041 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 
     5101 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCTAGGGG 
     5161 GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT 
     5221 AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC 
     5281 CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT 
     5341 TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA 
     5401 CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA TATATACTTT AGATTGATTT 
     5461 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 
     5521 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 
     5581 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 
     5641 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 
     5701 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 
     5761 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 
     5821 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 
     5881 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 
     5941 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 
     6001 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 
     6061 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 
     6121 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 
     6181 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 
     6241 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCATGC AT
//