LOCUS CD8-pTagRFP657-LOVpep_(T406A,_T407A,_I532A).xdna 5889 bp DNA circular 28/07/2017 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=Ligation of : pTagRFP657-LOVpep (T406A, T407A, I532A) .xdna [5139 nt] : (AgeI[666] / EcoRI[629]) to CD8mChFRB.xdna [742 nt] : (EcoRI[629] / AgeI[1375]) COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 2120..2551 /label=LOVpep (Optimal) /SerialCloner_Color=&hC0081A /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 1388..2086 /label=pTagRFP657 /SerialCloner_Color=&hA10524 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True 634..1375 /label=insert from CD8mChFRB.xdna /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from CD8mChFRB.xdna [742 nt] : (EcoRI[629] / AgeI[1375]) \ rep_origin 5165..5793 /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin 2815..3255 /label=F1 ori /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 3788..4579 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 3554..3750 /label=SV40 early promoter /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter 3322..3350 /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3601..3678 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 640..1360 /label=CD8 /SerialCloner_Color=&h0000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CAAGCTTCGa attCGGCGGC TCCCGCGCGC CTCCCCTCGC 661 GCCCGAGCTT CGAGCCAAGC AGCGTCCTGG GGAGCGCGTC ATGGCCTTAC CAGTGACCGC 721 CTTGCTCCTG CCGCTGGCCT TGCTGCTCCA CGCCGCCAGG CCGAGCCAGT TCCGGGTGTC 781 GCCGCTGGAT CGGACCTGGA ACCTGGGCGA GACAGTGGAG CTGAAGTGCC AGGTGCTGCT 841 GTCCAACCCG ACGTCGGGCT GCTCGTGGCT CTTCCAGCCG CGCGGCGCCG CCGCCAGTCC 901 CACCTTCCTC CTATACCTCT CCCAAAACAA GCCCAAGGCG GCCGAGGGGC TGGACACCCA 961 GCGGTTCTCG GGCAAGAGGT TGGGGGACAC CTTCGTCCTC ACCCTGAGCG ACTTCCGCCG 1021 AGAGAACGAG GGCTACTATT TCTGCTCGGC CCTGAGCAAC TCCATCATGT ACTTCAGCCA 1081 CTTCGTGCCG GTCTTCCTGC CAGCGAAGCC CACCACGACG CCAGCGCCGC GACCACCAAC 1141 ACCGGCGCCC ACCATCGCGT CGCAGCCCCT GTCCCTGCGC CCAGAGGCGT GCCGGCCAGC 1201 GGCGGGGGGC GCAGTGCACA CGAGGGGGCT GGACTTCGCC TGTGATATCT ACATCTGGGC 1261 GCCCTTGGCC GGGACTTGTG GAGTCCTTCT CCTGTCACTG GTTATCACCC TTTACTGCAA 1321 GCGGcTTAAG CGAAGAAGGA TCCCCGCCGC CGCCGCCGCC GCCGCCGCCG TCAGAccggt 1381 ggCCACCATG AGCGAGCTGA TcAccGAGAA CATGCACATG AAGCTGTACA TGGAGGGCAC 1441 CGTGAACAAC CACCACTTCA AGTGCACATC CGAGGGCGAA GGCAAGCCCT ACGAGGGCAC 1501 CCAGACCCAG AGAATCAAGG TGGTCGAGGG CGGCCCTCTC CCCTTCGCCT TCGACATCCT 1561 GGCTACCAGC TTCATGTACG GCAGTCACAC CTTCATCAAC CACACCCAGG GCATCCCCGA 1621 CTTCTGGAAG CAGTCCTTCC CTGAGGGCTT CACATGGGAG AGAGTCACCA CATACGAAGA 1681 CGGGGGCGTG CTGACCGCTA CCCAGGACAC CAGCCTCCAG GACGGCTGCC TCATCTACAA 1741 CGTCAAGATC AGAGGGGTGA ACTTCCCATC CAACGGCCCT GTGATGCAGA AGAAAACACT 1801 CGGCTGGGAG GCCCACACCG AGATGCTGTA CCCCGCTGAC GGCGGCCTGG AAGGCAGAAC 1861 CGCGCTGGCC CTGAAGCTCG TGGGCGGGGG CCACCTGATC TGCAACTTCA AGACCACATA 1921 CAGATCCAAG AAACCCGCTA AGAACCTCAA GATGCCCGGC GTCTACTATG TGGACTACAG 1981 ACTGGAAAGA ATCAAGGAGG CCGACAAAGA GACCTACGTC GAGCAGCACG AGGTGGCTGT 2041 GGCCAGATAC TGCGACCTCC CTAGCAAACT GGGGCACAAG CTTAATcggt acAAGcgGTA 2101 CAAGTCCGGA CTCAGATCTt tggctgctgc acttgaacgt attgagaaga actttgtcat 2161 tactgaccca agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt 2221 gacagaatat agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga 2281 aactgatcgc gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac 2341 tgttcagctg attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca 2401 gcctatgcga gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac 2461 tgagcatgtc cgagatgctg ccgagagaga ggcagtcatg ctggcgaaga aaactgcaga 2521 agagattgat aaagcggtgg atacctgggt gTAGtGCggc cGCGACTCTA GATCATAATC 2581 AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG 2641 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT 2701 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT 2761 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA 2821 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC 2881 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT 2941 TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA 3001 AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG 3061 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG 3121 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC 3181 TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA 3241 TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 3301 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 3361 AATGCTTCAA TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT 3421 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 3481 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 3541 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 3601 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 3661 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 3721 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT 3781 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 3841 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 3901 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 3961 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 4021 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 4081 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 4141 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 4201 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 4261 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC 4321 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 4381 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 4441 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 4501 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 4561 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC 4621 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA 4681 ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC 4741 TTCGCCCACC CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 4801 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT 4861 AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG 4921 GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC 4981 CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT 5041 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT 5101 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC 5161 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC 5221 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 5281 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA 5341 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 5401 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 5461 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 5521 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA 5581 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 5641 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 5701 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 5761 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG 5821 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC 5881 GCCATGCAT //