LOCUS       CD8-pTagRFP657-LOVpep_(T406A,_T407A,_I532A).xdna    5889 bp      DNA     circular   28/07/2017
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pTagRFP657-LOVpep (T406A, T407A, I532A) .xdna 
            [5139  nt] : (AgeI[666] / EcoRI[629]) to CD8mChFRB.xdna [742
              nt] : (EcoRI[629] / AgeI[1375])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2120..2551
                     /label=LOVpep (Optimal)
                     /SerialCloner_Color=&hC0081A
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1388..2086
                     /label=pTagRFP657
                     /SerialCloner_Color=&hA10524
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     634..1375
                     /label=insert from CD8mChFRB.xdna
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from CD8mChFRB.xdna [742  nt] : (EcoRI[629] / AgeI[1375]) \ 
     rep_origin      5165..5793
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      2815..3255
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3788..4579
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        3554..3750
                     /label=SV40 early promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        3322..3350
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3601..3678
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    640..1360
                     /label=CD8
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 
      601 CCGGACTCAG ATCTCGAGCT CAAGCTTCGa attCGGCGGC TCCCGCGCGC CTCCCCTCGC 
      661 GCCCGAGCTT CGAGCCAAGC AGCGTCCTGG GGAGCGCGTC ATGGCCTTAC CAGTGACCGC 
      721 CTTGCTCCTG CCGCTGGCCT TGCTGCTCCA CGCCGCCAGG CCGAGCCAGT TCCGGGTGTC 
      781 GCCGCTGGAT CGGACCTGGA ACCTGGGCGA GACAGTGGAG CTGAAGTGCC AGGTGCTGCT 
      841 GTCCAACCCG ACGTCGGGCT GCTCGTGGCT CTTCCAGCCG CGCGGCGCCG CCGCCAGTCC 
      901 CACCTTCCTC CTATACCTCT CCCAAAACAA GCCCAAGGCG GCCGAGGGGC TGGACACCCA 
      961 GCGGTTCTCG GGCAAGAGGT TGGGGGACAC CTTCGTCCTC ACCCTGAGCG ACTTCCGCCG 
     1021 AGAGAACGAG GGCTACTATT TCTGCTCGGC CCTGAGCAAC TCCATCATGT ACTTCAGCCA 
     1081 CTTCGTGCCG GTCTTCCTGC CAGCGAAGCC CACCACGACG CCAGCGCCGC GACCACCAAC 
     1141 ACCGGCGCCC ACCATCGCGT CGCAGCCCCT GTCCCTGCGC CCAGAGGCGT GCCGGCCAGC 
     1201 GGCGGGGGGC GCAGTGCACA CGAGGGGGCT GGACTTCGCC TGTGATATCT ACATCTGGGC 
     1261 GCCCTTGGCC GGGACTTGTG GAGTCCTTCT CCTGTCACTG GTTATCACCC TTTACTGCAA 
     1321 GCGGcTTAAG CGAAGAAGGA TCCCCGCCGC CGCCGCCGCC GCCGCCGCCG TCAGAccggt 
     1381 ggCCACCATG AGCGAGCTGA TcAccGAGAA CATGCACATG AAGCTGTACA TGGAGGGCAC 
     1441 CGTGAACAAC CACCACTTCA AGTGCACATC CGAGGGCGAA GGCAAGCCCT ACGAGGGCAC 
     1501 CCAGACCCAG AGAATCAAGG TGGTCGAGGG CGGCCCTCTC CCCTTCGCCT TCGACATCCT 
     1561 GGCTACCAGC TTCATGTACG GCAGTCACAC CTTCATCAAC CACACCCAGG GCATCCCCGA 
     1621 CTTCTGGAAG CAGTCCTTCC CTGAGGGCTT CACATGGGAG AGAGTCACCA CATACGAAGA 
     1681 CGGGGGCGTG CTGACCGCTA CCCAGGACAC CAGCCTCCAG GACGGCTGCC TCATCTACAA 
     1741 CGTCAAGATC AGAGGGGTGA ACTTCCCATC CAACGGCCCT GTGATGCAGA AGAAAACACT 
     1801 CGGCTGGGAG GCCCACACCG AGATGCTGTA CCCCGCTGAC GGCGGCCTGG AAGGCAGAAC 
     1861 CGCGCTGGCC CTGAAGCTCG TGGGCGGGGG CCACCTGATC TGCAACTTCA AGACCACATA 
     1921 CAGATCCAAG AAACCCGCTA AGAACCTCAA GATGCCCGGC GTCTACTATG TGGACTACAG 
     1981 ACTGGAAAGA ATCAAGGAGG CCGACAAAGA GACCTACGTC GAGCAGCACG AGGTGGCTGT 
     2041 GGCCAGATAC TGCGACCTCC CTAGCAAACT GGGGCACAAG CTTAATcggt acAAGcgGTA 
     2101 CAAGTCCGGA CTCAGATCTt tggctgctgc acttgaacgt attgagaaga actttgtcat 
     2161 tactgaccca agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt 
     2221 gacagaatat agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga 
     2281 aactgatcgc gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac 
     2341 tgttcagctg attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca 
     2401 gcctatgcga gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac 
     2461 tgagcatgtc cgagatgctg ccgagagaga ggcagtcatg ctggcgaaga aaactgcaga 
     2521 agagattgat aaagcggtgg atacctgggt gTAGtGCggc cGCGACTCTA GATCATAATC 
     2581 AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG 
     2641 AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT 
     2701 GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT 
     2761 TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA 
     2821 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC 
     2881 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT 
     2941 TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA 
     3001 AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG 
     3061 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG 
     3121 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC 
     3181 TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA 
     3241 TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 
     3301 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 
     3361 AATGCTTCAA TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT 
     3421 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     3481 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     3541 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     3601 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     3661 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     3721 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT 
     3781 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 
     3841 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 
     3901 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 
     3961 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 
     4021 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 
     4081 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 
     4141 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 
     4201 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 
     4261 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC 
     4321 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 
     4381 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 
     4441 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 
     4501 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 
     4561 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC 
     4621 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA 
     4681 ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC 
     4741 TTCGCCCACC CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 
     4801 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT 
     4861 AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG 
     4921 GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC 
     4981 CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT 
     5041 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT 
     5101 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC 
     5161 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC 
     5221 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 
     5281 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA 
     5341 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 
     5401 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 
     5461 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 
     5521 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA 
     5581 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 
     5641 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 
     5701 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 
     5761 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG 
     5821 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC 
     5881 GCCATGCAT
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