LOCUS       pAAV.Syn.Flex.GCaMP6f.WPRE.SV40\(\p2819)\Q        6247 bp    DNA     circular     16-OCT-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|640085995|
COMMENT     VNTDBDATE|640085995|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 ( p2819) Q|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 ( p2819) Q" 0 6247 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AatI" "AatII" "Acc65I" "AccI" "AclI" "Acu I" "AfeI" 
COMMENT         "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AlwNI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbsI" 
COMMENT         "BbvCI" "BcgI" "BciVI" "BclI" "BfrBI" "BfuAI" "BglI" "BglII" "BlpI" 
COMMENT         "BmgBI" "BmrI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" 
COMMENT         "BsaXI" "BseRI" "BseYI" "BsgI" "BsiEI" "BsiHKAI" "BsiWI" "BsmBI" "BsmI" 
COMMENT         "Bsp1286I" "BspDI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstXI" "BstYI" 
COMMENT         "BstZ17I" "Bsu36I" "BtgI" "BtgZ I" "BtsCI" "BtsI" "ClaI (m)" 
COMMENT         "ClaI (nm)" "DraI" "DraIII" "DrdI" "EaeI" "EagI" "EarI" "EciI" "EcoNI" 
COMMENT         "EcoO109I" "EcoRI" "EcoRV" "FseI" "FspI" "HaeII" "HincII" "HindIII" 
COMMENT         "HpaI" "I-SceI" "KasI" "KpnI" "MfeI" "MluI" "MscI" "MslI" "MspA1I" 
COMMENT         "NaeI" "NarI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "NspI" 
COMMENT         "PacI" "PciI" "PflFI" "PflMI" "PI-SceI" "PmeI" "PmlI" "PpuMI" "PshAI" 
COMMENT         "PsiI" "PspOMI" "PstI" "PvuI" "PvuII" "RsrII" "SacI" "SacII" "SalI" 
COMMENT         "SapI" "SbfI" "ScaI" "SexAI" "SfcI" "SfiI" "SfoI" "SgrAI" "SmaI" "SmlI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SrfI" "SspI" "StuI" "StyI" "SwaI" "Tth111I" 
COMMENT         "XbaI" "XcmI" "XhoI" "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6247 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AatI" "aggcct" 4 0 1 6236 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 459 0 2830 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1723 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 2072 0 2976 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AclI" "aacgtt" 3 0 2 4374 0 4747 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "Acu I" "ctgaag" 23 21 8 364 1 463 0 1242 1 
COMMENT            1318 1 1517 0 1561 1 4310 0 5322 1 0 "") 
COMMENT        #6=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 604 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "AflIII" "acrygt" 2 0 1 5864 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 3 59 0 3368 0 4976 0 
COMMENT             0 "") 
COMMENT        #11=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 3 1355 0 1532 0 1682 0 
COMMENT             0 "") 
COMMENT        #12=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 5455 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 202 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2586 0 4304 0 5550 0 0 
COMMENT             "") #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AseI" "attaat" 3 0 3 4801 0 6036 0 6095 0 0 
COMMENT             "") #17=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 6 29 0 40 0 2002 0 2986 0 
COMMENT             3382 0 3393 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 681 0 2188 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 7 252 0 588 0 1673 0 1723 
COMMENT             0 3792 0 5023 0 6120 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "BanII" "grgcyc" 6 0 4 202 0 2013 0 2730 0 
COMMENT             3762 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 740 0 2905 1 0 "") 
COMMENT        #25=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 435 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 5 380 0 758 1 2140 
COMMENT             0 2249 1 4438 1 0 "") 
COMMENT        #27=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 715 0 2205 1 2446 1 
COMMENT             4139 0 5666 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 900 0 1119 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BfuAI" "acctgc" 11 15 1 1266 1 0 "") 
COMMENT        #31=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 31 0 3398 0 3591 0 
COMMENT             4858 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BglII" "agatct" 2 0 2 185 0 2284 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 1 2012 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 1 2063 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 3481 0 4936 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1270 1 1765 1 4907 0 0 
COMMENT             "") 
COMMENT        #37=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 2 435 0 1841 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 4372 0 5240 0 5481 1 
COMMENT             5779 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 4 167 0 954 0 3259 0 3833 
COMMENT             0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 2 2092 0 3236 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 6 45 0 456 0 589 0 2827 0 
COMMENT             3379 0 4436 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1155 1 4910 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 3 622 1 3872 1 
COMMENT             6026 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BseRI" "gaggag" 17 15 4 646 0 1684 1 2502 1 
COMMENT             2563 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 524 0 1178 0 5560 0 0 
COMMENT             "") 
COMMENT        #46=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 5 954 1 1489 1 1586 0 
COMMENT             1819 1 1900 1 0 "") 
COMMENT        #47=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 7 268 0 2995 0 3562 0 
COMMENT             4458 0 4607 0 5530 0 5954 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 5 2013 0 2590 0 4308 0 
COMMENT             4393 0 5554 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 2 3056 0 3149 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 13 202 0 565 0 1298 0 
COMMENT             1547 0 1676 0 2013 0 2590 0 2730 0 2743 0 3762 0 4308 0 4393 0 5554 
COMMENT             0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BspDI" "atcgat" 3 0 4 884 0 2372 0 2967 0 
COMMENT             3230 0 0 "") #54=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 4136 0 5144 0 0 "") 
COMMENT        #56=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1266 1 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsrBI" "gagcgg" 4 4 4 3689 1 4134 1 5935 1 
COMMENT             6176 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 983 1 2624 1 4742 0 
COMMENT             4916 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 7 465 0 519 0 591 0 1558 
COMMENT             0 2889 0 3728 0 4891 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 848 0 1734 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 7 3 0 88 0 418 0 420 0 
COMMENT             448 0 3334 0 3419 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 1528 0 4307 0 5691 0 0 
COMMENT             "") #63=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 2 1821 0 2351 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 1186 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "BstYI" "rgatcy" 2 0 15 185 0 681 0 1944 0 
COMMENT             2087 0 2111 0 2188 0 2284 0 2939 0 3237 0 4329 0 4346 0 5114 0 5126 
COMMENT             0 5212 0 5223 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BtgI" "ccrygg" 2 0 6 387 0 2076 0 2178 0 2681 
COMMENT             0 2753 0 2877 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BtgZ I" "gcgatg" 17 21 3 1587 1 2679 1 3828 1 
COMMENT             0 "") 
COMMENT        #72=(CRSite (CStringList) "BtsCI" "ggatgnn" 8 6 13 241 0 317 1 355 0 875 
COMMENT             1 989 1 1202 1 1308 0 1437 0 1674 0 3514 1 4530 0 4817 0 4998 0 0 
COMMENT             "") 
COMMENT        #73=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 6 369 1 1501 1 3061 0 4557 
COMMENT             0 4577 1 6084 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "ClaI (m)" "gatcgat" 4 6 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "ClaI (nm)" "hatcgatd" 4 0 4 884 0 2372 0 2967 
COMMENT             0 3230 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "DraI" "tttaaa" 4 0 5 1239 0 3196 0 4398 0 
COMMENT             5090 0 5109 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 3836 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "DrdI" "gacnnnnnngtc" 8 0 3 2277 0 3880 0 5762 
COMMENT             0 0 "") 
COMMENT        #79=(CRSite (CStringList) "EaeI" "yggccr" 2 0 8 104 0 491 0 1637 0 2992 
COMMENT             0 3318 0 3447 0 4583 0 6025 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "EagI" "cggccg" 2 0 1 2992 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "EarI" "ctcttc" 8 11 8 862 0 922 0 1261 0 2338 
COMMENT             1 2909 0 3540 1 4177 1 5981 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "EciI" "ggcgga" 18 16 7 1466 0 1957 0 2201 0 
COMMENT             2701 0 4834 0 5662 0 5808 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 2 2343 0 6241 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 2 198 0 199 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 662 0 1228 0 2353 0 0 
COMMENT             "") #86=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2361 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "FspI" "tgcgca" 4 0 4 316 0 368 0 3581 0 4753 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 592 0 606 0 3678 0 3686 
COMMENT             0 5624 0 5994 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 928 0 2073 0 2977 0 
COMMENT             3135 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2365 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 928 0 3135 0 0 "") 
COMMENT        #93=(CRSite (CStringList) "I-SceI" "twgggataacagggtaat" 10 6 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "KasI" "ggcgcc" 2 0 1 588 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1727 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "MfeI" "caattg" 2 0 1 3144 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "MscI" "tggcca" 4 0 2 106 0 3320 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "MslI" "caynnnnrtg" 6 0 7 1355 0 1532 0 1682 0 
COMMENT             2158 0 4205 0 4564 0 4723 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 10 389 0 628 0 2714 0 
COMMENT              2863 0 2879 0 3531 0 4340 0 5281 0 5526 0 6044 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "NaeI" "gccggc" 4 0 4 521 0 593 0 2891 0 3730 
COMMENT              0 0 "") 
COMMENT        #102=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 589 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 2076 0 2178 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 4 519 0 591 0 2889 0 
COMMENT              3728 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 673 0 2143 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2992 0 0 "") 
COMMENT        #108=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #109=(CRSite (CStringList) "NsiI" "atgcat" 6 0 0 0 "") 
COMMENT        #110=(CRSite (CStringList) "NspI" "rcatgy" 6 0 1 5868 0 0 "") 
COMMENT        #111=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 2 3433 0 6231 0 0 "") 
COMMENT        #112=(CRSite (CStringList) "PciI" "acatgt" 2 0 1 5864 0 0 "") 
COMMENT        #113=(CRSite (CStringList) "PflFI" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #114=(CRSite (CStringList) "PflMI" "ccannnnntgg" 8 0 1 2618 0 0 "") 
COMMENT        #115=(CRSite (CStringList) "PI-SceI" 
COMMENT              "atctatgtcgggtgcggagaaagaggtaatgaaatggca" 16 12 0 0 "") 
COMMENT        #116=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #117=(CRSite (CStringList) "PmlI" "cacgtg" 4 0 1 954 0 0 "") 
COMMENT        #118=(CRSite (CStringList) "PpuMI" "rggwccy" 3 0 0 0 "") 
COMMENT        #119=(CRSite (CStringList) "PshAI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #120=(CRSite (CStringList) "PsiI" "ttataa" 4 0 2 3115 0 3961 0 0 "") 
COMMENT        #121=(CRSite (CStringList) "PspOMI" "gggccc" 2 0 1 198 0 0 "") 
COMMENT        #122=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 195 0 1133 0 0 "") 
COMMENT        #123=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3562 0 4607 0 0 "") 
COMMENT        #124=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 3531 0 6044 0 0 "") 
COMMENT        #125=(CRSite (CStringList) "RsrII" "cggwccg" 3 0 1 531 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2013 0 0 "") 
COMMENT        #127=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 390 0 2880 0 0 "") 
COMMENT        #128=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 2071 0 2975 0 0 "") 
COMMENT        #129=(CRSite (CStringList) "SapI" "gctcttc" 9 12 2 1261 0 5981 1 0 "") 
COMMENT        #130=(CRSite (CStringList) "SbfI" "cctgcagg" 7 0 0 0 "") 
COMMENT        #131=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4495 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "SexAI" "accwggt" 2 0 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "SfcI" "ctryag" 2 0 7 191 0 1129 0 2022 0 
COMMENT              3610 0 4730 0 5408 0 5599 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "SfoI" "ggcgcc" 4 0 1 590 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 1 591 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 31 0 42 0 2988 0 3384 0 
COMMENT              3395 0 0 "") 
COMMENT        #138=(CRSite (CStringList) "SmlI" "ctyrag" 2 0 5 2002 0 4351 0 5219 0 
COMMENT              5496 0 5758 0 0 "") 
COMMENT        #139=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 2 167 0 3259 0 0 "") 
COMMENT        #140=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") 
COMMENT        #141=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #142=(CRSite (CStringList) "SrfI" "gcccgggc" 5 0 4 31 0 42 0 3384 0 3395 
COMMENT              0 0 "") 
COMMENT        #143=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 4041 0 4171 0 0 "") 
COMMENT        #144=(CRSite (CStringList) "StuI" "aggcct" 4 0 1 6236 0 0 "") 
COMMENT        #145=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 6 497 0 938 0 1185 0 2076 
COMMENT              0 2178 0 2785 0 0 "") 
COMMENT        #146=(CRSite (CStringList) "SwaI" "atttaaat" 5 0 0 0 "") 
COMMENT        #147=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #148=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #149=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 0 0 "") 
COMMENT        #150=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2002 0 0 "") 
COMMENT        #151=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 29 0 40 0 2986 0 3382 0 
COMMENT              3393 0 0 "") 
COMMENT        #152=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 2347 0 4376 0 0 "")) 
COMMENT       (CObList 
COMMENT        #153=(CFSignal (CObList) "Amp(r)" 22 0 0 4188 5045 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #154=(CFSignal (CObList) "GCaMP6f" 4 0 1 831 2180 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #155=(CFSignal (CObList) "5' ITR" 34 0 0 1 167 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #156=(CFSignal (CObList) "3' ITR" 34 0 1 3258 3424 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #157=(CFSignal (CObList) "loxP" 21 0 0 784 817 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #158=(CFSignal (CObList) "loxP" 21 0 0 2301 2334 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #159=(CFSignal (CObList) "loxP2272" 21 0 0 688 721 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #160=(CFSignal (CObList) "loxP2272" 21 0 0 2197 2230 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "") 
COMMENT        #161=(CFSignal (CObList) "SV40 polyA signal" 25 0 0 2998 3229 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #162=(CFSignal (CObList) "synapsin promoter" 29 0 0 190 662 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #163=(CFSignal (CObList) "WPRE" 22 0 0 2376 2964 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                                             " N2: 2905 (on complementary strand)" 
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COMMENT                             "GAAGACNNNNNNN" "CTTCTGNNNNNNN") 
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COMMENT                             "CGANNNNNNTGCNNNNNNNNNNNNN" 
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COMMENT                              "BciVI: 5 sites") 12 11 "GTATCCNNNNNNN" 
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COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
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COMMENT                              "BglII: 2 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                             "CACGTC" "GTGCAG") 
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COMMENT                              "BmrI: 2 sites") 11 10 "ACTGGGNNNNNN" 
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COMMENT                              "BpmI: 3 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                                       #359=(CLinePar 
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COMMENT                             "CCTNAGC" "GGANTCG") 
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COMMENT                              "BpuEI: 4 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaAI: 4 sites") 3 3 "YACGTR" "RTGCAY") 
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COMMENT                              "BsaBI: 2 sites") 5 5 "GATNNNNATC" "CTANNNNTAG") 
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COMMENT                              "BsaHI: 6 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                                             " N2: 3872 (on complementary strand)" 
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COMMENT                              "BsaXI: 3 sites") 21 18 "ACNNNNNCTCCNNNNNNNNNNN" 
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COMMENT                                       #396=(CLinePar 
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COMMENT                              "BseRI: 4 sites") 16 14 "GAGGAGNNNNNNNNNNN" 
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COMMENT                                       #403=(CLinePar 
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COMMENT                              "BseYI: 3 sites") 1 5 "CCCAGC" "GGGTCG") 
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COMMENT                                       #409=(CLinePar 
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COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
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COMMENT                                       #414=(CLinePar 
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COMMENT                                       #415=(CLinePar 
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COMMENT                                       #416=(CLinePar 
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COMMENT                                       #417=(CLinePar 
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COMMENT                                       #418=(CLinePar 
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COMMENT                                       #419=(CLinePar 
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COMMENT                              "BsiEI: 7 sites") 4 2 "CGRYCG" "GCYRGC") 
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COMMENT                                       #423=(CLinePar 
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COMMENT                                       #424=(CLinePar 
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COMMENT                                       #425=(CLinePar 
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COMMENT                                       #426=(CLinePar 
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COMMENT                              "BsiHKAI: 5 sites") 5 1 "GWGCWC" "CWCGWG") 
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COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                                       #432=(CLinePar 
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COMMENT                             "GAATGCNN" "CTTACGNN") 
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COMMENT                              "BstXI: 1 site") 8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                              "BstYI: 15 sites") 1 5 "RGATCY" "YCTAGR") 
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COMMENT                              "BtgI: 6 sites") 1 5 "CCRYGG" "GGYRCC") 
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COMMENT                                             " N2: 2679 (on complementary strand)" 
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COMMENT                                             " N3: 3828 (on complementary strand)" 
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COMMENT                             "GCGATGNNNNNNNNNNNNNNN" "CGCTACNNNNNNNNNNNNNNN") 
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COMMENT                                       #545=(CLinePar 
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COMMENT                                             " N2: 317 (on complementary strand)" 
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COMMENT                                       #547=(CLinePar 
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COMMENT                                             " N4: 875 (on complementary strand)" 
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COMMENT                                             " N5: 989 (on complementary strand)" 
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COMMENT                                       #549=(CLinePar 
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COMMENT                                             " N6: 1202 (on complementary strand)" 
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COMMENT                                       #550=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #72# 7) 1 2 2 0 
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COMMENT                                       #551=(CLinePar 
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COMMENT                                       #552=(CLinePar 
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COMMENT                                       #553=(CLinePar 
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COMMENT                                             " N10: 3514 (on complementary strand)" 
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COMMENT                                       #555=(CLinePar 
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COMMENT                                              191) " N12: 4817 " 1) 
COMMENT                                       #556=(CLinePar 
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COMMENT                              "BtsCI: 13 sites") 7 5 "GGATGNNN" "CCTACNNN") 
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COMMENT                                             " N1: 369 (on complementary strand)" 
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COMMENT                                       #560=(CLinePar 
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COMMENT                                             " N2: 1501 (on complementary strand)" 
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COMMENT                                       #562=(CLinePar 
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COMMENT                                       #563=(CLinePar 
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COMMENT                                             " N5: 4577 (on complementary strand)" 
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COMMENT                              "BtsI: 6 sites") 8 6 "GCAGTGNNN" "CGTCACNNN") 
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COMMENT                                       #570=(CLinePar 
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COMMENT                                       #571=(CLinePar 
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COMMENT                              "ClaI (nm): 4 sites") 3 5 "HATCGATD" "DTAGCTAH") 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                              "EciI: 7 sites") 17 15 "GGCGGANNNNNNNNNNNN" 
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COMMENT                              "HaeII: 6 sites") 5 1 "RGCGCY" "YCGCGR") 
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COMMENT                              "KasI: 1 site") 1 5 "GGCGCC" "CCGCGG") 
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COMMENT                              "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") 
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COMMENT                              "MfeI: 1 site") 1 5 "CAATTG" "GTTAAC") 
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COMMENT                              "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT") 
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COMMENT                              "MslI: 7 sites") 5 5 "CAYNNNNRTG" "GTRNNNNYAC") 
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COMMENT                              "MspA1I: 10 sites") 3 3 "CMGCKG" "GKCGMC") 
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COMMENT                              "NaeI: 4 sites") 3 3 "GCCGGC" "CGGCCG") 
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COMMENT                              "NarI: 1 site") 2 4 "GGCGCC" "CCGCGG") 
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COMMENT                              "NcoI: 2 sites") 1 5 "CCATGG" "GGTACC") 
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COMMENT                                      "Georgia") 1 0.555556 0 
COMMENT                                     "{\\i Bst}YI (5126)" "@N (@S)" 5 0 0 0 -10 
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COMMENT                                      "Georgia") 1 0.555556 0 
COMMENT                                     "{\\i Bst}YI (5212)" "@N (@S)" 5 0 0 0 -10 
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COMMENT                                      "Georgia") 1 0.555556 0 
COMMENT                                     "{\\i Bst}YI (5223)" "@N (@S)" 5 0 0 0 -10 
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FEATURES             Location/Qualifiers
     misc_marker     4188..5045
                     /vntifkey="22"
                     /label=Amp(r)
     CDS             complement(831..2180)
                     /vntifkey="4"
                     /label=GCaMP6f
     repeat_region   1..167
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(3258..3424)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    784..817
                     /vntifkey="21"
                     /label=loxP
     misc_feature    2301..2334
                     /vntifkey="21"
                     /label=loxP
     misc_feature    688..721
                     /vntifkey="21"
                     /label=loxP2272
     misc_feature    2197..2230
                     /vntifkey="21"
                     /label=loxP2272
     polyA_signal    2998..3229
                     /vntifkey="25"
                     /label=SV40\polyA\signal
     promoter        190..662
                     /vntifkey="29"
                     /label=synapsin\promoter
     misc_marker     2376..2964
                     /vntifkey="22"
                     /label=WPRE
BASE COUNT     1350 a      1710 c      1592 g      1595 t 
ORIGIN
        1 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg 
       61 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta 
      121 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta 
      181 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag 
      241 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc 
      301 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg 
      361 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc 
      421 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac 
      481 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca 
      541 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc 
      601 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc 
      661 gaattcaagc tgctagcaag gatccccata acttcgtata aagtatccta tacgaagtta 
      721 tatcaaaata ggaagaccaa tgcttcacca tcgacccgaa ttgccaagca tcaccatcga 
      781 cccataactt cgtataatgt atgctatacg aagttatact agagctttca cttcgctgtc 
      841 atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc tgcttccctg 
      901 atcatttcat caacctcttc atctgttaac ttctctccaa ggtttgtcat cacgtggcga 
      961 agctctgctg cactgatgta gccattgcca tccttatcaa acacaccgaa cgcttctcta 
     1021 atttcttctt ccgtgtccct gtatttcatt tttcttgcca tcattgtcag gaactcaggg 
     1081 aagtcgattg tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct 
     1141 tctgtggggt tctgccccag agaccgcatc accgtcccca gctccttggt tgttattgtc 
     1201 ccatccccgt ccttgtcaaa tagggagaat tcctctttaa attctgcgat ctgctcttca 
     1261 gtcagttggt ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg 
     1321 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg 
     1381 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct 
     1441 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag 
     1501 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg 
     1561 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc accctcgccg 
     1621 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc 
     1681 ccggtgaaca gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc 
     1741 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg 
     1801 cgcttctcgt tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg gttgtcgggc 
     1861 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg 
     1921 ccgccgtcct cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg 
     1981 tcggccttga tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga 
     2041 cctgtcttat tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca 
     2101 tcgtcgtaca gatcccgacc catttgctgt ccaccagtca tgctagccat accatgatga 
     2161 tgatgatgat gagaacccat ggtggcggat cctagtataa cttcgtatag gatactttat 
     2221 acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg accctctagt 
     2281 ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag ttatgtccct 
     2341 cgaagaggtt cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 
     2401 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 
     2461 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 
     2521 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 
     2581 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 
     2641 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 
     2701 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 
     2761 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 
     2821 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 
     2881 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 
     2941 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgacccggg cggccgcttc 
     3001 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 
     3061 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 
     3121 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga 
     3181 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc 
     3241 ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa 
     3301 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 
     3361 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 
     3421 cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 
     3481 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 
     3541 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 
     3601 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     3661 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     3721 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 
     3781 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 
     3841 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     3901 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 
     3961 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     4021 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg 
     4081 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
     4141 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 
     4201 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 
     4261 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 
     4321 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 
     4381 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
     4441 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 
     4501 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 
     4561 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 
     4621 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 
     4681 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
     4741 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 
     4801 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 
     4861 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 
     4921 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 
     4981 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
     5041 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 
     5101 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 
     5161 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     5221 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     5281 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     5341 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 
     5401 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     5461 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     5521 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     5581 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     5641 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     5701 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     5761 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     5821 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 
     5881 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 
     5941 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 
     6001 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 
     6061 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 
     6121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 
     6181 aacaatttca cacaggaaac agctatgacc atgattacgc cagatttaat taaggcctta 
     6241 attaggc 
//
