LOCUS       pENN.AAV.CamKII.GCaMP6f.WPRE.SV40\(P3435)\Q        6485 bp    DNA     circular     27-MAY-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|699097187|
COMMENT     VNTDBDATE|699097187|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (P3435) Q|
COMMENT     VNTAUTHORNAME|Audrey Mazza|
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FEATURES             Location/Qualifiers
     CDS             1479..2828
                     /vntifkey="4"
                     /label=GCaMP6f
     misc_feature    156..1448
                     /vntifkey="21"
                     /label=a-CaMKII(1.3)
     misc_feature    5813..6480
                     /vntifkey="21"
                     /label=pUC\ori
     CDS             4805..5662
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3980..4286
                     /vntifkey="21"
                     /label=f1\Origin
     LTR             complement(3748..3888)
                     /vntifkey="19"
                     /label=R-ITR
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     misc_marker     2846..3433
                     /vntifkey="22"
                     /label=WPRE
     insertion_seq   3454..3692
                     /vntifkey="14"
                     /label=SV40\polyA
BASE COUNT     1564 a      1683 c      1741 g      1497 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt 
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt 
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt 
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact 
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca 
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc 
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat 
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc 
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat 
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct 
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc 
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga 
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac 
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc 
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa 
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat 
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc 
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa 
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt 
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga 
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca 
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg 
     1441 ggggcagctc tagagcggta ccggatccac ccgccaccat gggttctcat catcatcatc 
     1501 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg 
     1561 acgataagga tctcgccacc atggtcgact catcacgtcg taagtggaat aagacaggtc 
     1621 acgcagtcag agctataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca 
     1681 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg 
     1741 tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 
     1801 ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg 
     1861 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 
     1921 tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg 
     1981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     2041 gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 
     2101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 
     2161 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     2221 gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     2281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     2341 aggaggacgg caacatcctg gggcacaagc tggagtacaa cctgccggac caactgactg 
     2401 aagagcagat cgcagaattt aaagaggaat tctccctatt tgacaaggac ggggatggga 
     2461 caataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag 
     2521 cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc 
     2581 ctgagttcct gacaatgatg gcaagaaaaa tgaaatacag ggacacggaa gaagaaatta 
     2641 gagaagcgtt cggtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc 
     2701 gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca 
     2761 gggaagcaga catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga 
     2821 cagcgaagtg aaagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 
     2881 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     2941 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     3001 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     3061 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     3121 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     3181 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 
     3241 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     3301 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     3361 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     3421 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat 
     3481 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 
     3541 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 
     3601 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa 
     3661 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac 
     3721 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct 
     3781 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct 
     3841 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc 
     3901 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata 
     3961 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     4021 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     4081 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     4141 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg 
     4201 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     4261 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt 
     4321 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     4381 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat 
     4441 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 
     4501 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 
     4561 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 
     4621 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 
     4681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 
     4741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 
     4801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 
     4861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 
     4921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 
     4981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 
     5041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 
     5101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 
     5161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 
     5221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 
     5281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 
     5341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 
     5401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 
     5461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 
     5521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 
     5581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 
     5641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 
     5701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 
     5761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 
     5821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 
     5881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 
     5941 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 
     6001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 
     6061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 
     6121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 
     6181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 
     6241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 
     6301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 
     6361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 
     6421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 
     6481 catgt 
//
